EIF4A3 Antibody

Images

 
Western Blot: EIF4A3 Antibody [NBP1-85268] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: EIF4A3 Antibody [NBP1-85268] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: EIF4A3 Antibody [NBP1-85268] - Staining of human colon shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

EIF4A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIK
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EIF4A3 Protein (NBP1-85268PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EIF4A3 Antibody

  • ATP-dependent RNA helicase DDX48
  • ATP-dependent RNA helicase eIF4A-3
  • DDX48
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 48
  • DEAD box protein 48
  • DKFZp686O16189
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4AIII
  • eIF-4A-III
  • eIF4A-III
  • eukaryotic initiation factor 4A-III
  • Eukaryotic initiation factor 4A-like NUK-34
  • Eukaryotic translation initiation factor 4A isoform 3
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A3
  • hNMP 265
  • KIAA0111eukaryotic translation initiation factor 4A, isoform 3
  • MGC10862
  • NMP 265
  • NMP265
  • Nuclear matrix protein 265
  • NUK34

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4A3