eIF4A1 Antibody - BSA Free

Western Blot: eIF4A1 Antibody [NBP2-16302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A1 antibody diluted by 1:3000.

Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody [NBP2-16302] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-16302] - Human colon carcinoma, using EIF4A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation: eIF4A1 Antibody [NBP2-16302] - HepG2 whole cell extracts using 5 ug of EIF4A1 antibody. Western blot analysis was performed using EIF4A1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

eIF4A1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit eIF4A1 Antibody - BSA Free (NBP2-16302) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF4A1. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Chimpanzee (100%), Bovine (100%), Canine (100%), Xenopus (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for eIF4A1 Antibody - BSA Free

• ATP-dependent RNA helicase eIF4A-1
• DDX2Aeukaryotic initiation factor 4A-I
• EC 3.6.1
• EC 3.6.4.13
• EIF4A
• EIF-4A
• eIF-4A-I
• eIF4A-I
• eukaryotic initiation factor 4AI
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A, isoform 1
• eukaryotic translation initiation factor 4A1

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF4A1
• Uniprot:
  • P60842(Human)