eIF4A1 Antibody

Images

 
Western Blot: eIF4A1 Antibody [NBP2-16302] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody [NBP2-16302] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-16302] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

eIF4A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4A1 Antibody

  • ATP-dependent RNA helicase eIF4A-1
  • DDX2Aeukaryotic initiation factor 4A-I
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4A
  • EIF-4A
  • eIF-4A-I
  • eIF4A-I
  • eukaryotic initiation factor 4AI
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A, isoform 1
  • eukaryotic translation initiation factor 4A1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4A1
Uniprot