eIF4A1 Antibody (7C3) - BSA Free

Western Blot: eIF4A1 Antibody (7C3) [NBP3-15220] - Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate. All lanes: eIF4A1 Antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 47 KDa. Observed band size: 50 KDa.

Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody (7C3) [NBP3-15220] - Immunofluorescence staining of Hela Cells with at eIF4A1 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 7C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

eIF4A1 Antibody (7C3) - BSA Free Summary

• Description: Novus Biologicals Rabbit eIF4A1 Antibody (7C3) - BSA Free (NBP3-15220) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human eIF4A1. UniProt ID (P60842).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF4A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for eIF4A1 Antibody (7C3) - BSA Free

• ATP-dependent RNA helicase eIF4A-1
• DDX2Aeukaryotic initiation factor 4A-I
• EC 3.6.1
• EC 3.6.4.13
• EIF4A
• EIF-4A
• eIF-4A-I
• eIF4A-I
• eukaryotic initiation factor 4AI
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A, isoform 1
• eukaryotic translation initiation factor 4A1

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4A1