EIF3B Antibody (S09-2A9)

Western Blot: EIF3B Antibody (S09-2A9) [NBP3-15026] - Western blot detection of EIF3B in K562, Hela cell lysates using EIF3B Rabbit mAb (1:1000 diluted). Predicted band size: 93kDa. Observed band size: 117kDa.

Immunohistochemistry-Paraffin: EIF3B Antibody (S09-2A9) [NBP3-15026] - Immunohistochemistry of EIF3B in paraffin-embedded Human breast cancer tissue using EIF3B Rabbit mAb at dilution 1/20

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S09-2A9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

EIF3B Antibody (S09-2A9) Summary

• Description: Novus Biologicals Rabbit EIF3B Antibody (S09-2A9) (NBP3-15026) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human EIF3B (Uniprot#: P55884)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF3B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20-1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/5000
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for EIF3B Antibody (S09-2A9)

• eIF3 p110
• eIF3 p116
• eIF3b
• eIF-3-eta
• EIF3-P110
• EIF3-P116
• EIF3S9EIF3-ETA
• Eukaryotic translation initiation factor 3 subunit 9
• eukaryotic translation initiation factor 3 subunit B
• eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD)
• eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa
• eukaryotic translation initiation factor 3, subunit B
• MGC104664
• MGC131875
• Prt1 homolog
• PRT1hPrt1

Background

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF3B