EHD2 Antibody

Western Blot: EHD2 Antibody [NBP2-33283] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: EHD2 Antibody [NBP2-33283] - Staining of human cell line A-431 shows localization to plasma membrane.

Immunohistochemistry: EHD2 Antibody [NBP2-33283] - Immunohistochemical staining for EHD2 protein. The immunohistochemical results showing moderate expression in tumor cells. Endothelial cells (internal control) are positive in all staining. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174737), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: EHD2 Antibody [NBP2-33283] - Staining of human kidney shows moderate positivity in glomeruli.

Immunohistochemistry: EHD2 Antibody [NBP2-33283] - Immunohistochemical staining for EHD2 protein. The immunohistochemical results showing no expression in tumor cells. Endothelial cells (internal control) are positive in all staining. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174737), licensed under a CC-BY license.

Immunohistochemistry: EHD2 Antibody [NBP2-33283] - Immunohistochemical staining for EHD2 protein. The immunohistochemical results showing weak expression in tumor cells. Endothelial cells (internal control) are positive in all staining. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174737), licensed under a CC-BY license.

Immunohistochemistry: EHD2 Antibody [NBP2-33283] - Immunohistochemical staining for EHD2 protein. The immunohistochemical results showing strong expression in tumor cells. Endothelial cells (internal control) are positive in all staining. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174737), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EHD2 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: HDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGPDEAMEDGEEGSDDEAEWVVTKDKSKYDEIFYNLAPADGKLSGSKAKTWM
• Predicted Species: Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EHD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  EHD2 Protein (NBP2-33283PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for EHD2 Antibody

• EH domain containing 2
• EH domain-containing protein 2
• EH-domain containing 2
• FLJ96617
• PAST homolog 2
• PAST2

Background

EHD2 encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EHD2 Antibody (NBP2-33283)(1)

Publication using NBP2-33283

• Kim Y, Kim MH, Jeon S et al. Prognostic implication of histological features associated with EHD2 expression in papillary thyroid carcinoma. PLoS One 2017-03-30 [PMID: 28358874]

Bioinformatics

• Gene Symbol: EHD2
• Uniprot:
  • Q9NZN4(Human)