Myoferlin Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Myoferlin Antibody [NBP1-84694] - Staining in human urinary bladder and skeletal muscle tissues using NBP1-84694 antibody. Corresponding MYOF RNA-seq data are ...read more
Orthogonal Strategies: Western Blot: Myoferlin Antibody [NBP1-84694] - Analysis in human cell lines A-431 and HEK293 using Anti-MYOF antibody. Corresponding MYOF RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: Myoferlin Antibody [NBP1-84694] - Staining of human cell line U-251 MG shows localization to plasma membrane & vesicles. Antibody staining is shown in green.
Western Blot: Myoferlin Antibody [NBP1-84694] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Myoferlin Antibody [NBP1-84694] - Staining of human placenta shows moderate to strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Myoferlin Antibody [NBP1-84694] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Myoferlin Antibody [NBP1-84694] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Myoferlin Antibody [NBP1-84694] - Staining of human urinary bladder shows strong membranous positivity in urothelial cells.
Simple Western: Myoferlin Antibody [NBP1-84694] - Lane view shows a specific band for Myoferlin using 200 ug/mL of RT4 cell lysate and 4 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Myoferlin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYOF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western reported by internal validaton.
Control Peptide
Myoferlin Protein (NBP1-84694PEP)
Publications
Read Publications using NBP1-84694.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%). Reactivity reported in scientific literature (PMID: 24586247)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myoferlin Antibody

  • fer-1 (C.elegans)-like 3 (myoferlin)
  • FER1L3
  • fer-1-like 3, myoferlin (C. elegans)
  • fer-1-like 3, myoferlin
  • Fer-1-like protein 3
  • FLJ36571
  • FLJ90777
  • KIAA1207
  • myoferlin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Myoferlin Antibody (NBP1-84694)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Myoferlin Antibody (NBP1-84694)

Discover related pathways, diseases and genes to Myoferlin Antibody (NBP1-84694). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Myoferlin Antibody (NBP1-84694)

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Blogs on Myoferlin

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Bioinformatics

Gene Symbol MYOF