PTRF Antibody - BSA Free

Western Blot: PTRF Antibody [NBP1-80220] - Sample Tissue: Mouse Lung Antibody Dilution: 1.0ug/ml

Immunohistochemistry-Paraffin: PTRF Antibody [NBP1-80220] - Mouse vessel and adipocytes (skeletal muscle), 5 ug/ml.

Western Blot: PTRF Antibody [NBP1-80220] - NIH/3T3 cell lysate, Antibody Titration: 0.625ug/ml

Western Blot: PTRF Antibody [NBP1-80220] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml

Western Blot: PTRF Antibody [NBP1-80220] - Lanes: Lane 1: 50 ug mouse embryonic fibroblast lysate Lane 2: 50 ug mouse PTRF-KO embryonic fibroblast lysate Lane 3: 50 ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein Primary Antibody Dilution: 1:1000 Secondary Antibody: Donkey anti-Rabb

• Reactivity: Hu, Mu
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PTRF Antibody - BSA Free Summary

• Description: The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
• Immunogen: Synthetic peptide directed towards the middle region of mouse PTRF. Peptide sequence SEALPEKEGDELGEGERPEDDTAAIELSSDEAVEVEEVIEESRAERIKRS. The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAVIN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
  • Western Blot 1.0 ug/ml
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PTRF Antibody - BSA Free

• CAVIN
• cavin-1
• CGL4
• FLJ90031
• polymerase I and transcript release factor
• RNA polymerase I and transcript release factor
• TTF-I interacting peptide 12

Background

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTRF Antibody (NBP1-80220)(2)

Publications using NBP1-80220

• Murphy S, Zweyer M, Mundegar RR et al. Chemical crosslinking analysis of ?-dystroglycan in dystrophin-deficient skeletal muscle HRB Open Research 2018-05-30 [PMID: 35528858]
• Nighot PK, Leung L, Ma TY. Chloride channel ClC- 2 enhances intestinal epithelial tight junction barrier function via regulation of caveolin-1 and caveolar trafficking of occludin. Exp. Cell Res. 2017-03-01 [PMID: 28161538]

Bioinformatics

• Gene Symbol: CAVIN1
• Uniprot:
  • NP_033012(Mouse)