EGLN3/PHD3 Antibody (SR1441) - BSA Free

Western Blot-NBP3-22670-EGLN3/PHD3 Antibody (SR1441)-Analysis of PHD3 expression in (1) A549 cell lysate; (2) NIH/3T3 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clone: SR1441
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EGLN3/PHD3 Antibody (SR1441) - BSA Free Summary

• Description: Novus Biologicals Rabbit EGLN3/PHD3 Antibody (SR1441) - BSA Free (NBP3-22670) is a recombinant monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human EGLN3/PHD3 (Uniprot #: Q9H6Z9)
• Specificity: Detects endogenous levels of total EGLN3/PHD3
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EGLN3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EGLN3/PHD3 Antibody (SR1441) - BSA Free

• EC 1.14.11
• EC 1.14.11.-
• EGL nine (C.elegans) homolog 3
• egl nine homolog 3 (C. elegans)
• egl nine homolog 3
• egl nine-like protein 3 isoform
• EGLN3
• FLJ21620
• HIF prolyl hydroxylase 3
• HIFPH3 MGC125998
• HIFPH3
• HIF-PH3
• HIF-prolyl hydroxylase 3
• HPH-1
• HPH-3
• Hypoxia-inducible factor prolyl hydroxylase 3
• MGC125999
• pdh3
• PHD3
• Prolyl hydroxylase domain-containing protein 3

Background

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGLN3