EGFR Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EGFR Antigen ...read more
EGFR was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EGFR at approximately ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

EGFR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EGFR
Leu25-Ser647
Accession # Q9EP98
Specificity
Detects mouse EGFR in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) EGFR and less than 5% cross-reactivity with rhErbB2, rhErbB3, and rhErbB4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGFR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1280 in the following applications:

Publications
Read Publications using
AF1280 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGFR Antibody [Unconjugated]

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as Her1, ErbB1, or ErbB), ErbB2 (Neu, Her2), ErbB3 (Her3), and ErbB4 (Her4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Avivi, A. et al. (1991) Oncogene 6:673.
  4. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
  5. Reiter J.L. et al. (2001) Genomics 71:1.
  6. Xu, Y.H. et al. (1984) Nature 309:806.
  7. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  8. Yarden, Y. et al. (1987) Biochemistry 26:1434.
  9. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  10. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
  11. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
  12. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  13. Miettinen, P.J. et al. (1995) Nature 376:337.
  14. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGFR Antibody (AF1280)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(1)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1280 Applications Species
J Tintelnot, N Baum, C Schultheis, F Braig, M Trentmann, J Finter, W Fumey, P Bannas, B Fehse, K Riecken, K Schütze, C Bokemeyer, T Rösner, T Valerius, M Peipp, F Koch-Nolte, M Binder Nanobody-targeting of epidermal growth factor receptor (EGFR) ectodomain variants overcomes resistance to therapeutic EGFR antibodies Mol. Cancer Ther., 2019;0(0):. 2019 [PMID: 30824613] (Flow Cytometry, Mouse) Flow Cytometry Mouse
YL Corcino, JC Portillo, CS Subauste Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondii Sci Rep, 2019;9(1):669. 2019 [PMID: 30679495] (Western Blot, Mouse) Western Blot Mouse
C Cheng, L Deng, R Li The Immunogenicity and Anti-Tumor Efficacy of a Rationally Designed EGFR Vaccine Cell. Physiol. Biochem., 2018;46(1):46-56. 2018 [PMID: 29566364] (Western Blot, Mouse) Western Blot Mouse
PM Turunen, LM Louhivuori, V Louhivuori, JP Kukkonen, KE Åkerman Endocannabinoid Signaling in Embryonic Neuronal Motility and Cell-Cell Contact - Role of mGluR5 and TRPC3 Channels Neuroscience, 2018;0(0):. 2018 [PMID: 29438802] (ICC, Mouse) ICC Mouse
X Qing, Y Chinenov, P Redecha, M Madaio, JJ Roelofs, G Farber, PD Issuree, L Donlin, DR McIlwain, TW Mak, CP Blobel, JE Salmon iRhom2 promotes lupus nephritis through TNF-? and EGFR signaling J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29369823] (Western Blot, Mouse) Western Blot Mouse
Puehringer D, Orel N, Luningschror P, Subramanian N, Herrmann T, Chao M, Sendtner M EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons. Nat Neurosci, 2013;16(4):407-15. 2013 [PMID: 23416450]
Irvin DK, Jouanneau E, Duvall G T cells enhance stem-like properties and conditional malignancy in gliomas. PLoS ONE, 2010;5(6):e10974. 2010 [PMID: 20539758] (Flow Cytometry, Mouse) Flow Cytometry Mouse

Review for EGFR Antibody (AF1280) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1280:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/05/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23074208
SpeciesMouse

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FAQs for EGFR Antibody (AF1280) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1280

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Using EGF Protein from Novus Biologicals
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The Merlin protein belongs to the ezrin/radixin/moesin (ERM) family of tumour suppressor proteins. Encoded by the rather less imaginatively named Neurofibromin 2 (NF2) gene, it is thought to play a role in extracellular signal transduction, linking th...  Read full blog post.

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Anonymous
01/05/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol EGFR
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