Immunocytochemistry/ Immunofluorescence: EGFR Antibody (29.1) [NB120-10414] - Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were ...read more
Immunohistochemistry-Paraffin: EGFR Antibody (29.1) [NB120-10414] - Human Breast Carcinoma
Immunohistochemistry-Paraffin: EGFR Antibody (29.1) [NB120-10414] - Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor Receptor. From R. Robinson, Univ. of Iowa, College of Med., Dept. of Pathology, ...read more
Mice were immunized with paraformaldehyde-fixed carcinoma cell line A431, which contains 2 x 106 EGF receptors per cell.
Monoclonal Anti-EGF Receptor binds to a carbohydrate residue on the external portion of the receptor molecule in such a way that it does not affect the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to EGF
Cell Membrane and Secreted
Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
The receptor for Epidermal Growth Factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. It is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. EGF-R has an extracellular region which binds EGF and consequently mediates the initial response of cells to EGF and an intra- cellular region which posseses the tyrosine kinase activity. As a result of EGF binding to its specific receptor, there is increased DNA synthesis as well as other events such as cell proliferation, differentiation and repair of damaged epithelial tissue. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R). It is hypothesized that an inappropriate activation of the human erb-B gene either by truncation or overexpression plays a role in the development of the malignancy. This hypothesis is supported by studies which have shown an increased number of EGF-R in various malignant tumors. High levels of EGF-R have been identified in sarcomas, gliomas, gynecological, breast, bladder and lung tumors.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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