EGFR Antibody (29.1)


Immunocytochemistry/ Immunofluorescence: EGFR Antibody (29.1) [NB120-10414] - Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were more
Immunohistochemistry-Paraffin: EGFR Antibody (29.1) [NB120-10414] - Human Breast Carcinoma
Immunohistochemistry-Paraffin: EGFR Antibody (29.1) [NB120-10414] - Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor Receptor. From R. Robinson, Univ. of Iowa, College of Med., Dept. of Pathology, more

Product Details

Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P

Order Details

EGFR Antibody (29.1) Summary

Mice were immunized with paraformaldehyde-fixed carcinoma cell line A431, which contains 2 x 106 EGF receptors per cell.
Monoclonal Anti-EGF Receptor binds to a carbohydrate residue on the external portion of the receptor molecule in such a way that it does not affect the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to EGF
Cell Membrane and Secreted
Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:3000
  • ELISA 1:10000 (Indirect)
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IP: Use at an assay dependent dilution. WB: Detects a band of approximately 170 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
A-431 Lysate (NB820-59461)
Kidney Lysate (NB820-59231)
Esophagus Lysate (NB820-59214)
Read Publications using
NB120-10414 in the following applications:

  • WB
    1 publication

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
15mM Sodium Azide

Alternate Names for EGFR Antibody (29.1)

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1


The receptor for Epidermal Growth Factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. It is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. EGF-R has an extracellular region which binds EGF and consequently mediates the initial response of cells to EGF and an intra- cellular region which posseses the tyrosine kinase activity. As a result of EGF binding to its specific receptor, there is increased DNA synthesis as well as other events such as cell proliferation, differentiation and repair of damaged epithelial tissue. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R). It is hypothesized that an inappropriate activation of the human erb-B gene either by truncation or overexpression plays a role in the development of the malignancy. This hypothesis is supported by studies which have shown an increased number of EGF-R in various malignant tumors. High levels of EGF-R have been identified in sarcomas, gliomas, gynecological, breast, bladder and lung tumors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGFR Antibody (NB120-10414)(2)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: WB.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGFR Antibody (NB120-10414) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Using EGF Protein from Novus Biologicals
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Gene Symbol EGFR