EGFR Antibody (29.1)

Images

 
Immunocytochemistry/ Immunofluorescence: EGFR Antibody (29.1) [NB120-10414] - Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were ...read more
Immunohistochemistry-Paraffin: EGFR Antibody (29.1) [NB120-10414] - Human Breast Carcinoma
Immunohistochemistry-Paraffin: EGFR Antibody (29.1) [NB120-10414] - Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor Receptor. From R. Robinson, Univ. of Iowa, College of Med., Dept. of Pathology, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
29.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

EGFR Antibody (29.1) Summary

Immunogen
Mice were immunized with paraformaldehyde-fixed carcinoma cell line A431, which contains 2 x 106 EGF receptors per cell.
Epitope
Monoclonal Anti-EGF Receptor binds to a carbohydrate residue on the external portion of the receptor molecule in such a way that it does not affect the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to EGF
Localization
Cell Membrane and Secreted
Specificity
Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGFR
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:3000
  • ELISA 1:10000 (Indirect)
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IP: Use at an assay dependent dilution. WB: Detects a band of approximately 170 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
A-431 Lysate (NB820-59461)
Kidney Lysate (NB820-59231)
Esophagus Lysate (NB820-59214)
Publications
Read Publications using
NB120-10414 in the following applications:

  • WB
    1 publication

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for EGFR Antibody (29.1)

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

The receptor for Epidermal Growth Factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. It is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. EGF-R has an extracellular region which binds EGF and consequently mediates the initial response of cells to EGF and an intra- cellular region which posseses the tyrosine kinase activity. As a result of EGF binding to its specific receptor, there is increased DNA synthesis as well as other events such as cell proliferation, differentiation and repair of damaged epithelial tissue. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R). It is hypothesized that an inappropriate activation of the human erb-B gene either by truncation or overexpression plays a role in the development of the malignancy. This hypothesis is supported by studies which have shown an increased number of EGF-R in various malignant tumors. High levels of EGF-R have been identified in sarcomas, gliomas, gynecological, breast, bladder and lung tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGFR Antibody (NB120-10414)(2)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGFR Antibody (NB120-10414) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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The relationship between Ki67 and HIF-1 in cancer
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Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

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Bioinformatics

Gene Symbol EGFR
Entrez