EGF Antibody [Unconjugated]

Images

 
Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236‑EG), Recombinant Mouse EGF (Catalog # 2028‑EG) and Recombinant Rat EGF (Catalog # 3214‑EG). PVDF Membrane was probed with 0.1 ...read more
EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

EGF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EGF
Specificity
Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat EGF and 5% cross-reactivity with recombinant mouse EGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.04-0.08 ug/mL
Control
EGF Knockout HeLa Cell Lysate
Publications
Read Publications using
AF236 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody [Unconjugated]

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGF Antibody (AF236)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Development, ICC, IHC, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(2)
IHC
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF236 Applications Species
M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human) Neutralization Human
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012;378(1):72-80. 2012 [PMID: 22366959] (ELISA Development, Human) ELISA Development Human
Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88. 2007 [PMID: 17600311] (Neutralization, Human) Neutralization Human
Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells. Biol. Reprod., 2007;77(1):53-60. 2007 [PMID: 17392498] (ICC, Human) ICC Human
Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72. 2006 [PMID: 16505018] (Western Blot, Human) Western Blot Human
Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Eur J Vasc Endovasc Surg, 2006;31(5):516-22. 2006 [PMID: 16427789] (IHC, Human) IHC Human
Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Dev. Biol., 2004;266(2):223-37. 2004 [PMID: 14738873] (ICC, Human) ICC Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGF Antibody (AF236) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF236

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Bioinformatics Tool for EGF Antibody (AF236)

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Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

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Bioinformatics

Gene Symbol EGF