EGF Antibody [Unconjugated]

Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236‑EG), Recombinant Mouse EGF (Catalog # 2028‑EG) and Recombinant Rat EGF (Catalog # 3214‑EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND50 is typically 0.04-0.08 µg/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EGF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human EGF
• Specificity: Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat EGF and 5% cross-reactivity with recombinant mouse EGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EGF
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.04-0.08 ug/mL
• Control:

  EGF Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody [Unconjugated]

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGF Antibody (AF236)(7)

Publications using AF236

• M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human)
• Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012;378(1):72-80. 2012 [PMID: 22366959] (ELISA Development, Human)
• Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88. 2007 [PMID: 17600311] (Neutralization, Human)
• Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells. Biol. Reprod., 2007;77(1):53-60. 2007 [PMID: 17392498] (ICC, Human)
• Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72. 2006 [PMID: 16505018] (Western Blot, Human)
• Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Eur J Vasc Endovasc Surg, 2006;31(5):516-22. 2006 [PMID: 16427789] (IHC, Human)
• Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Dev. Biol., 2004;266(2):223-37. 2004 [PMID: 14738873] (ICC, Human)

Bioinformatics

• Gene Symbol: EGF