EGF Antibody [Unconjugated]

Images

 
Recombinant Mouse EGF (Catalog # 2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ...read more
EGF was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) at 5 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EGF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse EGF
Specificity
Detects mouse EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human EGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.02-0.06 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2028 in the following applications:

Publications
Read Publications using
AF2028 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody [Unconjugated]

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha , amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1217 amino acid (aa) mouse EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature mouse EGF shares 70% and 77% aa sequence identity with mature human and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Gray, A. et al. (1983) Nature 303:722.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGF Antibody (AF2028)(6)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Neutralization.


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IHC
(3)
IHC-Fr
(1)
Neutralization
(3)
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Mouse
(6)
Transgenic Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF2028 Applications Species
JO Lamontagne, H Zhang, AM Zeid, K Strittmatt, AD Rocha, T Williams, S Zhang, AG Marneros Transcription factors AP-2alpha and AP-2beta regulate distinct segments of the distal nephron in the mammalian kidney Nature Communications, 2022-04-25;13(1):2226. 2022-04-25 [PMID: 35468900] (IHC, Mouse) IHC Mouse
AG Marneros Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron Cell Reports, 2021-01-12;34(2):108616. 2021-01-12 [PMID: 33440155] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
A critical role for NF2 and the Hippo pathway in branching morphogenesis Nat Commun, 2016-08-02;7(0):12309. 2016-08-02 [PMID: 27480037] (Neutralization, Mouse) Neutralization Mouse
Ohkubo , Hirotoki, Ito , Yoshiya, Minamino , Tsutomu, Eshima , Koji, Kojo , Ken, Okizaki , Shin-ich, Hirata , Mitsuhir, Shibuya , Masabumi, Watanabe , Masahiko, Majima , Masataka VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury. PLoS ONE, 2014-08-27;9(8):e105533. 2014-08-27 [PMID: 25162491] (IHC, Mouse) IHC Mouse
Ohkubo , Hirotoki, Ito , Yoshiya, Minamino , Tsutomu, Mishima , Toshiaki, Hirata , Mitsuhir, Hosono , Kanako, Shibuya , Masabumi, Yokomizo , Takehiko, Shimizu , Takao, Watanabe , Masahiko, Majima , Masataka Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment. FASEB J, 2013-04-29;27(8):3132-43. 2013-04-29 [PMID: 23629862] (IHC-Fr, Neutralization, Mouse) IHC-Fr, Neutralization Mouse
Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages. J. Clin. Invest., 2011-04-25;121(5):1692-708. 2011-04-25 [PMID: 21519141] (Neutralization, Mouse) Neutralization Mouse

Review for EGF Antibody (AF2028) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2028:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-Fr Mouse 01/06/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23074208
SpeciesMouse

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Secondary Antibodies

 

Isotype Controls

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Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

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Verified Customer
01/06/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol EGF