EGF Antibody (262930)

• Reactivity: Mu
• Applications: WB, ELISA(Cap)
• Clone: 262930
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

EGF Antibody (262930) Summary

• Immunogen: E. coli-derived recombinant mouse EGF
  Asn977-Arg1029
  Accession # P01132
• Specificity: Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha , rhNRG1-beta 1, or rhTGF-alpha .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: EGF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Mouse EGF Antibody (Catalog # MAB2028)
  ELISA Detection: Mouse EGF Biotinylated Antibody (Catalog # BAF2028)
  Standard: Recombinant Mouse EGF (Catalog # 2028-EG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody (262930)

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal Growth Factor (EGF) is the prototypic member of a family of growth factors that have EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGF Antibody (MAB2028)(1)

Publication using MAB2028

• Ohkubo , Hirotoki, Ito , Yoshiya, Minamino , Tsutomu, Mishima , Toshiaki, Hirata , Mitsuhir, Hosono , Kanako, Shibuya , Masabumi, Yokomizo , Takehiko, Shimizu , Takao, Watanabe , Masahiko, Majima , Masataka Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment. FASEB J, 2013-04-29;27(8):3132-43. 2013-04-29 [PMID: 23629862] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: EGF
• Uniprot:
  • P01132()