EEA1 Antibody (5G3.2C2)

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Product Details

Summary
Product Discontinued
View other related EEA1 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-36569
    • Availability
      Product Discontinued

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EEA1 Antibody (5G3.2C2) Summary

Immunogen
Partial recombinant human EEA1 protein (between amino acids 50-300) [UniProt Q15075]
Localization
Cytoplasm, Early endosome membrane
Marker
Early Endosome Marker
Predicted Species
Rat (95%), Porcine (95%), Bat (93%), Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EEA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular) 2.5 ug/million cells
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot
Control
EEA1 Overexpression Lysate
Publications
Read Publication using
NBP2-36569 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EEA1 Antibody (5G3.2C2)

  • early endosome antigen 1
  • early endosome antigen 1, 162kD
  • early endosome-associated protein
  • EEA1
  • Endosome-associated protein p162
  • MST105
  • MSTP105
  • ZFYVE2
  • ZFYVE2MST105
  • Zinc finger FYVE domain-containing protein 2

Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EEA1 Antibody (NBP2-36569)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

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FAQs for EEA1 Antibody (NBP2-36569) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional EEA1 Products

Bioinformatics Tool for EEA1 Antibody (NBP2-36569)

Discover related pathways, diseases and genes to EEA1 Antibody (NBP2-36569). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for EEA1 Antibody (NBP2-36569)

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Pathways for EEA1 Antibody (NBP2-36569)

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PTMs for EEA1 Antibody (NBP2-36569)

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Research Areas for EEA1 Antibody (NBP2-36569)

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Blogs on EEA1.

Understanding EEA1's Role in Membrane Endosome Fusion
EEA1, or Early Endosome Antigen 1 is a Rab5 effector essential for early endocytic membrane fusion. The EEA1 antibody is used in membrane trafficking and chaperone studies, and as an endosome marker. We at Novus Biologicals have a comprehensive antibo...  Read full blog post.

EEA1 Antibodies in Endosomal Transport and Signalling Studies
EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have ...  Read full blog post.

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Bioinformatics

Gene Symbol EEA1
Entrez
Uniprot