EBI3 Antibody (607201) [Allophycocyanin]

KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-27/IL-35 EBI3 Subunit APC-conjugated Monoclonal Antibody (Catalog # IC6456A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 607201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

EBI3 Antibody (607201) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit
  Arg21-Lys229 (predicted)
  Accession # Q14213
• Specificity: Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EBI3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBI3 Antibody (607201) [Allophycocyanin]

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-Induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4⁺ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBI3 Antibody (IC6456A)(3)

Publications using IC6456A

• Hong‑Xia Wang, Xia Kang, Shuai Chu, Haixia Li, Xin Li, Xiaofeng Yin et al. Dysregulated ICOS+ proinflammatory and suppressive regulatory T cells�in patients with rheumatoid arthritis Experimental and Therapeutic Medicine 2018-08-24 [PMID: 30233732] (Flow Cytometry, Human)
• Hong‑Xia Wang, Xia Kang, Shuai Chu, Haixia Li, Xin Li, Xiaofeng Yin et al. Dysregulated ICOS+ proinflammatory and suppressive regulatory T cells�in patients with rheumatoid arthritis Experimental and Therapeutic Medicine 8/24/2018 [PMID: 30233732]
• IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells. Biochem Biophys Res Commun, 2012-11-12;430(1):364-9. 2012-11-12 [PMID: 23154182] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • Q14213(Human)