EBI3 Antibody (5P10D3)

Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: EBI3 Antibody (5P10D3) [NBP2-03943] - Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 2 ug/10^6 cells.

• Reactivity: Mu
• Applications: WB, ELISA, Flow
• Clone: 5P10D3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

EBI3 Antibody (5P10D3) Summary

• Immunogen: Full-length recombinant mouse EBI3 protein
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: EBI3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-5 ug/ml
  • ELISA
  • Flow Cytometry 1-3ug/10^6cells
• Application Notes: Use in ELISA reported in scientific literature (PMID: 30660991).

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for EBI3 Antibody (5P10D3)

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBI3 Antibody (NBP2-03943)(1)

Publication using NBP2-03943

• Ma N, Fang Y, Xu R et al. Ebi3 promotes T- and B-cell division and differentiation via STAT3. Mol. Immunol. Jan 17 2019 [PMID: 30660991] (ELISA, Mouse)

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • O35228(Mouse)