EBI3 Antibody (15k8D10)

Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.

Tissue Culture Substratum: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, TCS
• Clone: 15k8D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

EBI3 Antibody (15k8D10) Summary

• Immunogen: A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.
• Specificity: The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. This clone has been tested to be specific to the EBI3 subunit of the target protein. Clone 15K8D10 tests as being specific to the EBI3 subunit.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EBI3
• Purity: Protein G purified
• Endotoxin Note: < 0.06 EU/ug

Applications/Dilutions

• Dilutions:
  • Western Blot 3-5ug/ml~
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Tissue Culture Substratum
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID 27681506).

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for EBI3 Antibody (15k8D10)

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

IL-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, IL-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which IL-35 is also specifically produced. IL-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBI3 Antibody (NBP2-27362)(10)

Publications using NBP2-27362

• Liu J, Hao S, Chen X et al. Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion. Nat Commun. 2019 Oct 10 [PMID: 31601798] (ICC/IF, Human)
• Larousserie F, Kebe D, Huynh T et al. Evidence for IL-35 Expression in Diffuse Large B-Cell Lymphoma and Impact on the Patient's Prognosis Front Oncol Jun 28 2019 [PMID: 31316915] (IHC-P, Human)
• Javvadi LR, Parachuru VPB, Milne TJ et al. Expression of IL33 and IL35 in oral lichen planus. Arch. Dermatol. Res. Apr 9 2018 [PMID: 29633015] (IHC-P)
• Ma Y, Chen L, Xie G et al. Elevated level of interleukin-35 in colorectal cancer induces conversion of T cells into iTr35 by activating STAT1/STAT3. Oncotarget. 2016 Nov 08 [PMID: 27682874] (IHC, Human)
• Zhao Z, Chen X, Hao S et al. Increased interleukin-35 expression in tumor-infiltrating lymphocytes correlates with poor prognosis in patients with breast cancer Cytokine Sep 24 2016 [PMID: 27681506] (IHC, Human)
• Horlad H, Ma C, Yano H et al. An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma. Cancer Sci. Nov 1 2016 [PMID: 27564404] (IHC, Human)
• Long J, Guo H, Cui S et al. IL-35 expression in hepatocellular carcinoma cells is associated with tumor progression Oncotarget. Jul 19 2016 [PMID: 27329841] (IHC, Human)
• Fu YP, Yi Y, Cai XY et al. Overexpression of interleukin-35 associates with hepatocellular carcinoma aggressiveness and recurrence after curative resection. Br. J. Cancer. 2016 Mar 29 [PMID: 27002937] (IHC-P, Human)
• Mao H, Gao W, Ma C et al. Human placental trophoblasts express the immunosuppressive cytokine IL-35. Hum Immunol. 2013 Jul [PMID: 23619469] (IHC-P, Human)
  
  Details:
  IHC (P), Fig 1: first-trimester human placentae.
• Wang Z, Liu JQ, Liu Z et al. Tumor-derived IL-35 promotes tumor growth by enhancing myeloid cell accumulation and angiogenesis. J Immunol. 2013 Mar 1 [PMID: 23345334] (IHC-P, Human)
  
  Details:
  IHC (P), Fig 1: human large B cell lymphoma, nasopharyngeal carcinoma, skin, melanoma, lymph node metastatic melanoma.

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • NP_000873.2(Human)