EBI3 Antibody (15k8D10) - BSA Free


Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.
Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, NULL
BSA Free
1.0 mg/ml

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EBI3 Antibody (15k8D10) - BSA Free Summary

A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.
The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. Clone 15K8D10 tests as being specific to the EBI3 subunit of the target protein.
IgG1 Kappa
Protein G purified
Endotoxin Note
< 0.06 EU/ug
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 31601798)
  • Immunohistochemistry reported in scientific literature (PMID 27681506)
  • Immunohistochemistry-Paraffin 5ug/ml
  • Tissue Culture Substratum
  • Western Blot 3-5ug/ml~
Read Publications using
NBP2-27362 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1 ml PBS
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for EBI3 Antibody (15k8D10) - BSA Free

  • cytokine receptor
  • EBI3
  • EBI-3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 beta
  • IL-27 subunit beta
  • IL27B
  • IL-27B
  • IL35 EBI3
  • IL-35 EBI3
  • interleukin-27 subunit beta


IL-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, IL-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which IL-35 is also specifically produced. IL-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for EBI3 Antibody (NBP2-27362)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-P.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-27362 Applications Species
Ito T, Tanaka T, Nakamaru K et al. Interleukin-35 promotes the differentiation of regulatory T cells and suppresses Th2 response in IgG4-related type 1 autoimmune pancreatitis J. Gastroenterol. 2020-05-06 [PMID: 32377945]
Liu J, Hao S, Chen X et al. Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion. Nat Commun. 2019-10-10 [PMID: 31601798] (ICC/IF, Human) ICC/IF Human
Larousserie F, Kebe D, Huynh T et al. Evidence for IL-35 Expression in Diffuse Large B-Cell Lymphoma and Impact on the Patient's Prognosis Front Oncol 2019-06-28 [PMID: 31316915] (IHC-P, Human) IHC-P Human
Javvadi LR, Parachuru VPB, Milne TJ et al. Expression of IL33 and IL35 in oral lichen planus. Arch. Dermatol. Res. 2018-04-09 [PMID: 29633015] (IHC-P) IHC-P
Ma Y, Chen L, Xie G et al. Elevated level of interleukin-35 in colorectal cancer induces conversion of T cells into iTr35 by activating STAT1/STAT3. Oncotarget. 2016-11-08 [PMID: 27682874] (IF/IHC, Human) IF/IHC Human
Zhao Z, Chen X, Hao S et al. Increased interleukin-35 expression in tumor-infiltrating lymphocytes correlates with poor prognosis in patients with breast cancer Cytokine 2016-09-24 [PMID: 27681506] (IF/IHC, Human) IF/IHC Human
Horlad H, Ma C, Yano H et al. An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma. Cancer Sci. 2016-11-01 [PMID: 27564404] (IF/IHC, Human) IF/IHC Human
Long J, Guo H, Cui S et al. IL-35 expression in hepatocellular carcinoma cells is associated with tumor progression Oncotarget. 2016-07-19 [PMID: 27329841] (IF/IHC, Human) IF/IHC Human
Fu YP, Yi Y, Cai XY et al. Overexpression of interleukin-35 associates with hepatocellular carcinoma aggressiveness and recurrence after curative resection. Br. J. Cancer. 2016-03-29 [PMID: 27002937] (IHC-P, Human) IHC-P Human
Mao H, Gao W, Ma C et al. Human placental trophoblasts express the immunosuppressive cytokine IL-35. Hum Immunol. 2013-07-01 [PMID: 23619469] (IHC-P, Human)

IHC (P), Fig 1: first-trimester human placentae.
IHC-P Human
Show All 11 Publications.

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Gene Symbol EBI3