EBI3 Antibody (15k8D10) - BSA Free

Images

 
Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.
Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, TCS
Clone
15k8D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

EBI3 Antibody (15k8D10) - BSA Free Summary

Immunogen
A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.
Specificity
The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. This clone has been tested to be specific to the EBI3 subunit of the target protein. Clone 15K8D10 tests as being specific to the EBI3 subunit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EBI3
Purity
Protein G purified
Endotoxin Note
< 0.06 EU/ug
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Tissue Culture Substratum
  • Western Blot 3-5ug/ml~
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 27681506). Use in ICC/IF was reported in scientific literature (PMID: 31601798).
Publications
Read Publications using
NBP2-27362 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EBI3 Antibody (15k8D10) - BSA Free

  • cytokine receptor
  • EBI3
  • EBI-3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 beta
  • IL-27 subunit beta
  • IL27B
  • IL-27B
  • IL35 EBI3
  • IL-35 EBI3
  • interleukin-27 subunit beta

Background

IL-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, IL-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which IL-35 is also specifically produced. IL-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-27362
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, TCS
Supplier Logo

Publications for EBI3 Antibody (NBP2-27362)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-P
(5)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-27362 Applications Species
Ito T, Tanaka T, Nakamaru K et al. Interleukin-35 promotes the differentiation of regulatory T cells and suppresses Th2 response in IgG4-related type 1 autoimmune pancreatitis J. Gastroenterol. May 6 2020 [PMID: 32377945]
Liu J, Hao S, Chen X et al. Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion. Nat Commun. 2019 Oct 10 [PMID: 31601798] (ICC/IF, Human) ICC/IF Human
Larousserie F, Kebe D, Huynh T et al. Evidence for IL-35 Expression in Diffuse Large B-Cell Lymphoma and Impact on the Patient's Prognosis Front Oncol Jun 28 2019 [PMID: 31316915] (IHC-P, Human) IHC-P Human
Javvadi LR, Parachuru VPB, Milne TJ et al. Expression of IL33 and IL35 in oral lichen planus. Arch. Dermatol. Res. Apr 9 2018 [PMID: 29633015] (IHC-P) IHC-P
Ma Y, Chen L, Xie G et al. Elevated level of interleukin-35 in colorectal cancer induces conversion of T cells into iTr35 by activating STAT1/STAT3. Oncotarget. 2016 Nov 08 [PMID: 27682874] (IHC, Human) IHC Human
Zhao Z, Chen X, Hao S et al. Increased interleukin-35 expression in tumor-infiltrating lymphocytes correlates with poor prognosis in patients with breast cancer Cytokine Sep 24 2016 [PMID: 27681506] (IHC, Human) IHC Human
Horlad H, Ma C, Yano H et al. An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma. Cancer Sci. Nov 1 2016 [PMID: 27564404] (IHC, Human) IHC Human
Long J, Guo H, Cui S et al. IL-35 expression in hepatocellular carcinoma cells is associated with tumor progression Oncotarget. Jul 19 2016 [PMID: 27329841] (IHC, Human) IHC Human
Fu YP, Yi Y, Cai XY et al. Overexpression of interleukin-35 associates with hepatocellular carcinoma aggressiveness and recurrence after curative resection. Br. J. Cancer. 2016 Mar 29 [PMID: 27002937] (IHC-P, Human) IHC-P Human
Mao H, Gao W, Ma C et al. Human placental trophoblasts express the immunosuppressive cytokine IL-35. Hum Immunol. 2013 Jul [PMID: 23619469] (IHC-P, Human)

Details:
IHC (P), Fig 1: first-trimester human placentae.
IHC-P Human
Show All 11 Publications.

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Video Protocols

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Bioinformatics

Gene Symbol EBI3
Uniprot