Dynactin Subunit 1/DCTN1 Antibody

Images

 
Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Sample (30 ug of whole cell lysate) A: JurKat 5% SDS PAGE, antibody diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Analysis of methanol-fixed HeLa, using DCTN1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an ...read more
Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Paraffin-embedded Rat brain. DCTN1 antibody [C3], C-term diluted at 1:500.
Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Whole cell lysate treated with DCTN1 Antibody, diluted at 1:1000 with 7.5% SDS PAGE gel.
Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Paraffin-embedded OVCA, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Dynactin Subunit 1/DCTN1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DCTN1. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dynactin Subunit 1/DCTN1 Antibody

  • DAP-150
  • DCTN1
  • DP-150
  • dynactin 1 (p150, Glued (Drosophila) homolog)
  • Dynactin 1
  • Dynactin Subunit 1
  • glued homolog, Drosophila)
  • HMN7B
  • p135
  • p150 Glued (Drosophila) homolog
  • p150-glued

Background

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for Dynactin Subunit 1/DCTN1 Antibody (NBP1-30880)

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Bioinformatics

Gene Symbol DCTN1
Entrez