dynactin 4 Antibody - Azide and BSA Free

Western Blot: dynactin 4 Antibody - Azide and BSA Free [NBP3-23532] - NBP3-23532 at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 57 kDa

Immunohistochemistry-Paraffin: dynactin 4 Antibody - Azide and BSA Free [NBP3-23532] - Immunochemical staining of human dynactin 4 in human pancreas with NBP3-23532 at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: dynactin 4 Antibody - Azide and BSA Free [NBP3-23532] - Immunochemical staining of human dynactin 4 in human kidney with NBP3-23532 at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: dynactin 4 Antibody [NBP3-23532] - DCTN4 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL NBP3-23532 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23532, at 1:100 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size :57 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

dynactin 4 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human dynactin 4 fragmenty.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCTN4
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.0
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for dynactin 4 Antibody - Azide and BSA Free

• dynactin 4 (p62)
• dynactin p62 subunit
• dynactin subunit 4
• Dynactin subunit p62

Background

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN4