Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

Western Blot: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.

Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Human Ovary tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Human breast tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Human Kidney tissue using anti-TYRO3 mouse monoclonal antibody.

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Human lung tissue using anti-TYRO3 mouse monoclonal antibody.

Immunoprecipitation: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Lane 1: Negative Control Lane 2: NBP1-48039 Lane 3: NBP1-48040 Lane 4: NBP1-48041 Lane 5: NBP1-48042 Lane 6: NBP1-48043 Lane 7: NBP1-48044 Lane 8: NBP1-48045

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: OTI2C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free (NBP1-48040) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TYRO3 (NP_006284) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TYRO3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:500
• Theoretical MW: 96.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Receptor protein tyrosine kinases (RPTKs) are a class of transmembrane signaling molecules. They have been shown to regulate signaling cascades that control synaptic function in the central nervous system (CNS). Tyro3 is one of three RPTKs belonging to the Axl/UFO subfamily, that also includes Axl and Mer. Tyro3 is the most commonly expressed of these receptors in the CNS. It may be involved in the cell adhesion process, and is commonly found in adult tissues with expression mostly in brain, testis, vascular and lymphatic tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYRO3