DRAM Antibody - BSA Free

Western Blot: DRAM Antibody [NBP1-76759] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: DRAM NBP1-76758 (0.5 ug/mL), DRAM NBP1-76759 (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: DRAM Antibody [NBP1-76759] - Western blot analysis of DRAM in 293 cell lysate with DRAM antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: DRAM Antibody - BSA Free [NBP1-76759] - Validation of DRAM in Mouse Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling DRAM with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry: DRAM Antibody - BSA Free [NBP1-76759] - Validation of DRAM in Mouse Liver Tissue. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-DRAM antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: DRAM Antibody - BSA Free [NBP1-76759] - Validation of DRAM in Human Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver tissue labeling DRAM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry: DRAM Antibody - BSA Free [NBP1-76759] - Validation of DRAM in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DRAM antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DRAM Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DRAM Antibody - BSA Free (NBP1-76759) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human DRAM. The immunogen is located within amino acids 30-80 of DRAM. Amino Acid Sequence: LPYISDTGTTPPESGI
• Specificity: Human DRAM has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (128aa, 14kD). Mouse DRAM also has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (132aa, 15kD). Rat DRAM has only one isoform (238aa, 26kD). NBP1-76759 can detect both human isoforms , and also detect mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DRAM1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2-2.5 ug/mL
  • Immunohistochemistry-Paraffin 2-2.5 ug/ml
  • Western Blot 1-2 ug/mL
• Theoretical MW: 14, 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  DRAM Peptide (NBP1-76759PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for DRAM Antibody - BSA Free

• Damage-regulated autophagy modulator
• DNA damage-regulated autophagy modulator protein 1
• DNA-damage regulated autophagy modulator 1
• DRAMFLJ11259

Background

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DRAM1
• Uniprot:
  • AAH18435(Human)