E2F-4 Antibody

Images

 
Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Immunohistochemistry: E2F-4 Antibody [NBP1-21374] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: E2F-4 Antibody [NBP1-21374] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. NBP1-21374 used for WB at 0.1 mcg/ml ...read more
Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-E2F4 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela cells.goat anti-human MCM6 (NB100-251) and rabbit anti-human E2F4 (NBP1-21374). image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM7 (NB100-252) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink in Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms), ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela Cells. goat anti-human ATM (NB100-271) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: E2F-4 Antibody [NBP1-21374] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC5 (NB100-235) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

E2F-4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 363 and 413 of human E2F transcription factor 4 using the numbering given in entry NP_001941.2 (GeneID 1874).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
E2F4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NBP1-21374.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for E2F-4 Antibody

  • E2F transcription factor 4, p107/p130-binding
  • E2F4
  • E2F-4
  • E2F-4p107/p130-binding protein
  • transcription factor E2F4

Background

E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E2F-4 Antibody (NBP1-21374)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol E2F4
Entrez
Uniprot