DPPA2 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: DPPA2 Antibody [NBP2-98808] - Immunochemical staining of human DPPA2 in human brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

Immunocytochemistry/Immunofluorescence: DPPA2 Antibody [NBP2-98808] - Immunofluorescence staining of DPPA2 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98808 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DPPA2 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human DPPA2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DPPA2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry-Paraffin 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for DPPA2 Antibody - Azide and BSA Free

• CT100
• developmental pluripotency associated 2
• developmental pluripotency-associated protein 2
• DPPA2
• ECSA
• PESCRG1
• PESCRG1cancer/testis antigen 100
• Pluripotent embryonic stem cell-related gene 1 protein

Background

DPPA2, also known as Developmental pluripotency-associated protein 2, is a 33.8 kDa, 298 amino acid protein that is involved in binding to target gene promoters, such as SYCE1 and NKX2-5. Diseases such as epithelial ovarian cancer and lung cancer have been researched with this protein. The protein interacts with other proteins such as DPPA4, FXR2, MCM10, DVL2, and AP2M1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPPA2