DPPA4 Antibody [Unconjugated]

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DPPA4 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3730) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

DPPA4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse DPPA4
Asn125-Gly296
Accession # Q8CCG4
Specificity
Detects mouse DPPA4 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DPPA4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DPPA4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF3730 in the following applications:

  • 2 publications
  • ICC
    2 publications
  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPA4 Antibody [Unconjugated]

  • developmental pluripotency associated 4
  • developmental pluripotency-associated protein 4
  • DPPA4
  • FLJ10713,2410091M23Rik

Background

DPPA4 is a 33 kDa molecule that is expressed specifically in the early embryo, uncommitted cells of the germ line, and embryonic stem cells and is used as a marker of pluripotent cells. Mouse DPPA4 is 296 amino acids in length and contains an SAP (SAF-A/B, ACINUS, PIAS) putative DNA-binding domain. Two potential isoforms exist with deletions of amino acids 104‑120 and 69‑120, respectively. In the region used as an immunogen, mouse DPPA4 shows 57% amino acid sequence identity with human and 87% identity with rat DPPA4 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DPPA4 Antibody (AF3730)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ChIP, ICC, IHC.


Filter By Application
ChIP
(2)
ICC
(2)
IHC
(2)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3730 Applications Species
L Vanheer, J Song, N De Geest, A Janiszewsk, I Talon, C Provenzano, T Oh, J Chappell, V Pasque Tox4 modulates cell fate reprogramming J. Cell. Sci., 2019;0(0):. 2019 [PMID: 31519808] (ICC, Mouse) ICC Mouse
JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance BMC Biol., 2018;16(1):104. 2018 [PMID: 30236109] (IHC, Mouse) IHC Mouse
RH Klein, PY Tung, P Somanath, HJ Fehling, PS Knoepfler Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer Stem Cell Res, 2018;31(0):83-94. 2018 [PMID: 30031967] (ChIP, Mouse) ChIP Mouse
K Adachi, W Kopp, G Wu, S Heising, B Greber, M Stehling, MJ Araúzo-Bra, ST Boerno, B Timmermann, M Vingron, HR Schöler Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency Cell Stem Cell, 2018;0(0):. 2018 [PMID: 29910149] (IHC, Mouse) IHC Mouse
M Sone, N Morone, T Nakamura, A Tanaka, K Okita, K Woltjen, M Nakagawa, JE Heuser, Y Yamada, S Yamanaka, T Yamamoto Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency Cell Metab., 2017;25(5):1103-1117.e6. 2017 [PMID: 28467928] (ICC, Mouse) ICC Mouse
Tung P, Varlakhanova N, Knoepfler P Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. Stem Cells, 2013;31(11):2330-42. 2013 [PMID: 23963736] (ChIP, Mouse) ChIP Mouse

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Bioinformatics

Gene Symbol DPPA4
Uniprot