Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis using the Biotin conjugate of NB100-56521. Detection of Dnmt3a using NB100-55782 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either ...read more
Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate (Novus, 40212) using Dnmt3a antibody at 1 ug/ml.
Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - WB for Dnmt3a in mouse brain cells. Image from verified customer review.
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for DNMT3A Antibody (NB100-56521). (Showing 1 - 1 of 1 FAQ).
I ordered the Dnmt3a Antibody (64B814.1) and would like to use it in IF and eventually intracellular FACS staining. I wanted to know which conditions did you uses in ICC/IF since it is written in the data sheet that it has been tested for this application.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Go Ahead! Make My DNA DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re... Read full blog post.