Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-56521. An intracellular stain was performed on HeLa cells with DNMT3A antibody (64B814.1) ...read more
Mouse reactivity reported in scientific literature (PMID: 16631596).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
Alternate Names for DNMT3A Antibody (64B814.1)
DNA (cytosine-5-)-methyltransferase 3 alpha
DNA (cytosine-5)-methyltransferase 3A
DNA cytosine methyltransferase 3A2
DNA Methyltransferase 3 Alpha
DNA methyltransferase HsaIIIA
DNA MTase HsaIIIA
Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes. References 1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2
2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L.,... Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080
3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G.,... Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916
4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D.,... Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for DNMT3A Antibody (NB100-56521). (Showing 1 - 1 of 1 FAQs).
I ordered the Dnmt3a Antibody (64B814.1) and would like to use it in IF and eventually intracellular FACS staining. I wanted to know which conditions did you uses in ICC/IF since it is written in the data sheet that it has been tested for this application.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Go Ahead! Make My DNA DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re... Read full blog post.
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