DNMT3A Antibody (64B814.1)

Images

 
Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-56521. An intracellular stain was performed on HeLa cells with DNMT3A antibody (64B814.1) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow-IC, ICC/IF
Clone
64B814.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DNMT3A Antibody (64B814.1) Summary

Immunogen
This DNMT3A Antibody (64B814.1) was raised against bacteria expressed HIS-tag recombinant mouse Dnmt3a.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Flow (Intracellular)
Control
DNMT3A Overexpression Lysate
DNMT3A Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-56521 in the following applications:

Publications
Read Publications using
NB100-56521 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16631596).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for DNMT3A Antibody (64B814.1)

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA Methyltransferase 3 Alpha
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA
  • TBRS

Background

Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.

References

1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L.,... Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G.,... Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D.,... Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3A Antibody (NB100-56521)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Mouse
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Review for DNMT3A Antibody (NB100-56521) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56521:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot DNMT3A NB100-56521
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reviewed by:
Anonymous
WB Mouse 07/11/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from homogenized mouse brain cells
SpeciesMouse

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio2000 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-mouse IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7076
Secondary Concentration1:10K

Details

Detection NotesCaptured using X-rays (10 sec exposure) with Phototope-HRP Western Blot Detection System

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB100-56521). (Showing 1 - 1 of 1 FAQs).

  1. I ordered the Dnmt3a Antibody (64B814.1) and would like to use it in IF and eventually intracellular FACS staining. I wanted to know which conditions did you uses in ICC/IF since it is written in the data sheet that it has been tested for this application.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56521AF350
Alexa Fluor 405 NB100-56521AF405
Alexa Fluor 488 NB100-56521AF488
Alexa Fluor 532 NB100-56521AF532
Alexa Fluor 594 NB100-56521AF594
Alexa Fluor 647 NB100-56521AF647
Alexa Fluor 700 NB100-56521AF700
Alexa Fluor 750 NB100-56521AF750
Biotin NB100-55782
DyLight 350 NB100-56521UV
DyLight 405 NB100-56521V
DyLight 488 NB100-56521G
DyLight 550 NB100-56521R
DyLight 594 NB100-56521DL594
DyLight 650 NB100-56521C
DyLight 680 NB100-56521FR
DyLight 755 NB100-56521IR
FITC NB100-56521F
HRP NB100-56521H
Janelia Fluor 549 NBP2-33216JF549
Janelia Fluor 646 NBP2-33216JF646

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Blogs on DNMT3A.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Recent Reviews

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Anonymous
07/11/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol DNMT3A