DNMT3A Antibody (64B814.1)


Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis using the Biotin conjugate of NB100-56521. Detection of Dnmt3a using NB100-55782 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either ...read more
Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate (Novus, 40212) using Dnmt3a antibody at 1 ug/ml.
Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - WB for Dnmt3a in mouse brain cells. Image from verified customer review.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
0.5 mg/ml

DNMT3A Antibody (64B814.1) Summary

This antibody was raised against bacteria expressed HIS-tag recombinant mouse Dnmt3a.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


Reviewed Applications
Read 1 Review rated 5
NB100-56521 in the following application:

Read Publication using
NB100-56521 in the following applications:

  • WB
    1 publication

Alternate Names for DNMT3A Antibody (64B814.1)

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • Dnmt3a
  • DNMT3A2
  • EC
  • M.HsaIIIA


Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3A Antibody (NB100-56521)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

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Reviews for DNMT3A Antibody (NB100-56521) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56521:
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Images Ratings Applications Species Date Details
Western Blot DNMT3A NB100-56521
reviewed by:
WB Mouse 07/11/2014


ApplicationWestern Blot
Sample TestedLysate from homogenized mouse brain cells


Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio2000 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-mouse IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7076
Secondary Concentration1:10K


Detection NotesCaptured using X-rays (10 sec exposure) with Phototope-HRP Western Blot Detection System

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB100-56521). (Showing 1 - 1 of 1 FAQ).

  1. I ordered the Dnmt3a Antibody (64B814.1) and would like to use it in IF and eventually intracellular FACS staining. I wanted to know which conditions did you uses in ICC/IF since it is written in the data sheet that it has been tested for this application.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-56521AF405
Alexa Fluor (R) 488 Labeled NB100-56521AF488
Alexa Fluor (R) 647 Labeled NB100-56521AF647
Alexa Fluor (R) 700 Labeled NB100-56521AF700
Allophycocyanin Labeled NB100-56521APC
Biotin Labeled NB100-55782
DyLight 350 Labeled NB100-56521UV
DyLight 405 Labeled NB100-56521V
DyLight 405LS Labeled NB100-56521V3
DyLight 488 Labeled NB100-56521G
DyLight 550 Labeled NB100-56521R
DyLight 650 Labeled NB100-56521C
DyLight 680 Labeled NB100-56521FR
DyLight 755 Labeled NB100-56521IR
HRP Labeled NB100-56521H
PE Labeled NB100-56521PE
PerCP Labeled NB100-56521PCP

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Blogs on DNMT3A.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol DNMT3A

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