DNMT3A Antibody (64B1446) [Allophycocyanin]

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Flow Cytometry: DNMT3A Antibody (64B1446) [Allophycocyanin] [NB120-13888APC] - An intracellular stain was performed on Jurkat cells with DNMT3A Antibody (64B1446) NB120-13888APC (blue) and a matched isotype control ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [Allophycocyanin] [NB120-13888APC] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [Allophycocyanin] [NB120-13888APC] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
64B1446
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

DNMT3A Antibody (64B1446) [Allophycocyanin] Summary

Immunogen
This DNMT3A antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DNMT3A Antibody (64B1446) [Allophycocyanin]

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for DNMT3A Antibody (NB120-13888APC) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-13888AF350
Alexa Fluor 405 NB120-13888AF405
Alexa Fluor 488 NB120-13888AF488
Alexa Fluor 532 NB120-13888AF532
Alexa Fluor 594 NB120-13888AF594
Alexa Fluor 647 NB120-13888AF647
Alexa Fluor 700 NB120-13888AF700
Alexa Fluor 750 NB120-13888AF750
Allophycocyanin NB120-13888APC
Allophycocyanin/Cy7 NB120-13888APCCY7
DyLight 350 NB120-13888UV
DyLight 405 NB120-13888V
DyLight 488 NB120-13888G
DyLight 550 NB120-13888R
DyLight 594 NB120-13888DL594
DyLight 650 NB120-13888C
DyLight 680 NB120-13888FR
DyLight 755 NB120-13888IR
FITC NB120-13888F
HRP NB120-13888H
Janelia Fluor 549 NB120-13888JF549
Janelia Fluor 646 NB120-13888JF646
PE NB120-13888PE
PE/Atto594 NB120-13888PEATT594
PE/Cy5.5 NB120-13888PECY55
PE/Cy7 NB120-13888PECY7

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Bioinformatics Tool for DNMT3A Antibody (NB120-13888APC)

Discover related pathways, diseases and genes to DNMT3A Antibody (NB120-13888APC). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on DNMT3A.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Bioinformatics

Gene Symbol DNMT3A