Dnmt2 Antibody

Images

 
Western Blot: Dnmt2 Antibody [NB200-616] - Homogenized mouse brain cells. Image from verified customer review.
Western Blot: Dnmt2 Antibody [NB200-616] - analysis of Dnmt2 in mouse ES cell lysate using Dnmt2 antibodyat 2 ug/ml.
Western Blot: Dnmt2 Antibody [NB200-616] - Western blot analysis for Dnmt2a using NB200-616 at 2 ug/ml dilution against 10 ug of mouse testis lysates. A protein band of approximate mol. wt. of 42 kDa is detected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dnmt2 Antibody Summary

Immunogen
This antibody was raised against synthetic peptides corresponding amino acids 39-53 and 361-376 of mouse Dnmt2.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRDMT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
Application Notes
Western Blot: Detects a band of approximately 42 kDa.
Positive Control
Testis Lysate (NB820-59672)
Dnmt2 Lysate (NBL1-17263)
Reviewed Applications
Read 1 Review rated 5
using
NB200-616 in the following application:

Publications
Read Publication using
NB200-616 in the following applications:

  • WB
    1 publication

Reactivity Notes

. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Dnmt2 Antibody

  • DMNT2
  • DNA (cytosine-5-)-methyltransferase 2
  • DNA (cytosine-5)-methyltransferase-like protein 2
  • DNA methyltransferase homolog HsaIIP
  • DNA methyltransferase-2
  • DNA MTase homolog HsaIIP
  • DNMT2
  • EC 2.1.1
  • EC 2.1.1.29
  • M.HsaIIP
  • MHSAIIP
  • PUMET
  • RNMT1
  • tRNA (cytosine-5-)-methyltransferase
  • tRNA aspartic acid methyltransferase 1

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dnmt2 Antibody (NB200-616)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for Dnmt2 Antibody (NB200-616) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB200-616:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Dnmt2 NB200-616
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reviewed by:
Anonymous
WB Mouse 07/17/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from homogenized mouse brain cells
SpeciesMouse

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio1000 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7074
Secondary Concentration1:20K

Details

Detection NotesCaptured using X-rays (20 sec exposure) with Phototope-HRP Western Blot Detection System

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Dnmt2 Antibody (NB200-616) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Dnmt2 Antibody (NB200-616)

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Research Areas for Dnmt2 Antibody (NB200-616)

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Blogs on Dnmt2

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/17/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TRDMT1
Entrez