Cubilin Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Cubilin at approximately 500 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cubilin ...read more
Cubilin was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Sheep Anti-Human/Mouse/Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) at 1 µg/mL ...read more
Western blot shows lysates of human, mouse, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Cubilin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Cubilin
Ser36-Gly126
Accession # O60494
Specificity
Detects human Cubilin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CUBN
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 1-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3700 in the following applications:

Publications
Read Publications using
AF3700 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cubilin Antibody [Unconjugated]

  • cubilin
  • cubilin (intrinsic factor-cobalamin receptor)
  • Cubilin
  • CUBN
  • Cubulin
  • gp280
  • IFCR
  • MGA1

Background

Cubilin is a 460 kDa protein that is a component of a multiligand endocytic receptor complex, which also contains amnionless, megalin and LRP2. The receptor complex is expressed in several absorptive epithelia, including kidney proximal tubule, yolk sac, and the small intestine. Human Cubilin is synthesized as a 3623 residue precursor with a signal peptide, an amino-terminal domain, eight EGF-like repeats and 27 Cub domains. Co-expression with amnionless is required for the membrane anchoring, ER export and internalizing action of Cubilin. Within the region used as the immunogen, human, mouse and rat Cubilin share approximately 76% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cubilin Antibody (AF3700)(2)

Review for Cubilin Antibody (AF3700) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3700:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Mouse 12/19/2014
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Summary

ApplicationWestern Blot
Sample TestedKidney tissue homogenates
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio0.2 ug/ml

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/

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FAQs for Cubilin Antibody (AF3700) (0)

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Recent Reviews

4
5
0
4
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1
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Anonymous
12/19/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CUBN
Uniprot