Dnmt2 Antibodies
We offer Dnmt2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each Dnmt2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Dnmt2 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Dnmt2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Dnmt2 polyclonal antibodies and browse our Dnmt2 monoclonal antibody catalog.
Alternate Names for Dnmt2 Antibodies
anti-Dnmt2 antibody, anti-TRDMT1 antibody, anti-DMNT2 antibody, anti-DNA (cytosine-5)-methyltransferase-like protein 2 antibody, anti-DNA (cytosine-5-)-methyltransferase 2 antibody, anti-DNA methyltransferase homolog HsaIIP antibody, anti-DNA methyltransferase-2 antibody, anti-DNA MTase homolog HsaIIP antibody, anti-EC 2.1.1 antibody, anti-EC 2.1.1.29 antibody, anti-M.HsaIIP antibody, anti-MHSAIIP antibody, anti-PUMET antibody, anti-RNMT1 antibody, anti-tRNA (cytosine-5-)-methyltransferase antibody, anti-tRNA aspartic acid methyltransferase 1 antibody
6 Results for "dnmt2" in Primary Antibodies
![Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-102B1259-2-Western-Blot-NB200-587-img0002.jpg "Western Blot: Dnmt2 Antibody (102B1259.2) [NB200-587] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml.")
![Flow Cytometry: Dnmt2 Antibody (102B1259.2) [NB200-587] - An intracellular stain was performed on Jurkat cells with Dnmt2 Antibody [102B1259.2] NB200-587 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/Dnmt2-Antibody-102B1259-2-Flow-Cytometry-NB200-587-img0003.jpg)
![Western Blot: Dnmt2 Antibody [NB200-616] - Homogenized mouse brain cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Western-Blot-NB200-616-img0003.jpg "Western Blot: Dnmt2 Antibody [NB200-616] - Homogenized mouse brain cells. Image from verified customer review.")
![Western Blot: Dnmt2 Antibody [NB200-616] - analysis of Dnmt2 in mouse ES cell lysate using Dnmt2 antibodyat 2 ug/ml.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Western-Blot-NB200-616-img0002.jpg)
![Western Blot: Dnmt2 Antibody [NB200-616] - Western blot analysis for Dnmt2a using NB200-616 at 2 ug/ml dilution against 10 ug of mouse testis lysates. A protein band of approximate mol. wt. of 42 kDa is detected.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Western-Blot-NB200-616-img0001.jpg)
![Western Blot: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lane 4: HCT 116 cell lysate Lane 5: U-2 OS cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32275) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32275_rabbit-dnmt2-mab-psh03-43-27820241631637.jpg "Western Blot: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lane 4: HCT 116 cell lysate Lane 5: U-2 OS cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32275) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32275) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32275_rabbit-dnmt2-mab-psh03-43-29820241492682.jpg)
![Immunocytochemistry/ Immunofluorescence: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Immunocytochemistry analysis of MCF7 cells labeling Dnmt2 with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32275_rabbit-dnmt2-mab-psh03-43-2920241192376.jpg)
![Flow Cytometry: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Flow cytometric analysis of MCF7 cells labeling Dnmt2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32275, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32275_rabbit-dnmt2-mab-psh03-43-29202416453221.jpg)
![Western Blot: Dnmt2 Antibody [NBP3-18663] - Figure 2. Western blot analysis using the antibody directed against Dnmt2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Dnmt2 , diluted 1:1000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Western-Blot-NBP3-18663-img0001.jpg "Western Blot: Dnmt2 Antibody [NBP3-18663] - Figure 2. Western blot analysis using the antibody directed against Dnmt2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Dnmt2 , diluted 1:1000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right.")
![ELISA: Dnmt2 Antibody [NBP3-18663] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Dnmt2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:21,000.](http://images.novusbio.com/fullsize/Dnmt2 Antibody-ELISA-NBP3-18663-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Dnmt2 Antibody [NBP2-48670] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48670-img0003.jpg "Immunocytochemistry/Immunofluorescence: Dnmt2 Antibody [NBP2-48670] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")
![Immunohistochemistry-Paraffin: Dnmt2 Antibody [NBP2-48670] - Staining of human stomach, lower shows strong cytoplasmic and additional nucleolar positivity in subset of glandular cells.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Immunohistochemistry-Paraffin-NBP2-48670-img0002.jpg)
![Western Blot: Dnmt2 Antibody [NBP2-47483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Western-Blot-NBP2-47483-img0002.jpg "Western Blot: Dnmt2 Antibody [NBP2-47483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG")
![Immunohistochemistry: Dnmt2 Antibody [NBP2-47483] - Staining of human colon tissue. Shows cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Dnmt2-Antibody-Immunohistochemistry-NBP2-47483-img0001.jpg)