NSUN2 Antibody

Staining of human cell line A-431 shows positivity in nuclear membrane.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NSUN2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids:LKSEWQSMTQESFQESSVKELRRLEDQLAGLQQELAALALKQSSVAEEVGLLPQQIQAVRDDVESQFPAWISQFLARGGGGRVGLLQREEMQAQLRELESKILTHVAEMQGKSAREAAASLSLTLQKEGVIGVTEEQVHHIVKQALQRYSE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSUN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%). Reactivity reported in scientific literature (PMID: 21610090)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NSUN2 Antibody

• EC 2.1.1
• EC 2.1.1.29
• FLJ20303,5-methycytoisine methyltransferase
• hTrm4
• member 2
• Myc-induced SUN-domain-containing protein
• NOL1/NOP2/Sun domain family 2
• NOL1/NOP2/Sun domain family member 2
• NOP2/Sun domain family, member 2
• SAKIMisu
• Substrate of AIM1/Aurora kinase B
• TRM4MISU
• tRNA (cytosine-5-)-methyltransferase NSUN2
• tRNA (cytosine-5-)-methyltransferase
• tRNA methyltransferase 4 homolog

Background

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NSUN2 Antibody (NBP1-86878)(6)

Publications using NBP1-86878

• Lv XB, Liu L, Cheng C et al. SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer. Sci Rep 2015-12-10 [PMID: 26658802] (WB, IF/IHC, Human)
• Chaumet A, Wright GD, Seet SH et al. Nuclear envelope-associated endosomes deliver surface proteins to the nucleus. Nat Commun 2015-09-10 [PMID: 26356418] (ICC/IF, Human)
• Alsarraj J, Faraji F, Geiger TR et al. BRD4 Short Isoform Interacts with RRP1B, SIPA1 and Components of the LINC Complex at the Inner Face of the Nuclear Membrane. PLoS One 2013-01-01 [PMID: 24260471]
• Horn HF, Kim DI, Wright GD et al. A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. J Cell Biol 2013-09-30 [PMID: 24062341]
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]
• Gudise S, Figueroa RA, Lindberg R et al. Samp1 is functionally associated with the LINC complex and A-type lamina networks. J Cell Sci 2011-06-15 [PMID: 21610090] (ICC/IF)

Bioinformatics

• Gene Symbol: NSUN2