Dnmt2 Antibody (PSH03-43)

Western Blot: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lane 4: HCT 116 cell lysate Lane 5: U-2 OS cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32275) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32275) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Immunocytochemistry analysis of MCF7 cells labeling Dnmt2 with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt2 antibody (NBP3-32275) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: Dnmt2 Antibody (PSH03-43) [NBP3-32275] - Flow cytometric analysis of MCF7 cells labeling Dnmt2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32275, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PSH03-43
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Dnmt2 Antibody (PSH03-43) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human Dnmt2 aa 1-391 / 391. (Uniprot: O14717)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TRDMT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:2000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dnmt2 Antibody (PSH03-43)

• DMNT2
• DNA (cytosine-5-)-methyltransferase 2
• DNA (cytosine-5)-methyltransferase-like protein 2
• DNA methyltransferase homolog HsaIIP
• DNA methyltransferase-2
• DNA MTase homolog HsaIIP
• DNMT2
• EC 2.1.1
• EC 2.1.1.29
• M.HsaIIP
• MHSAIIP
• PUMET
• RNMT1
• tRNA (cytosine-5-)-methyltransferase
• tRNA aspartic acid methyltransferase 1

Background

tRNA aspartic acid methyltransferase 1 (TRDMT1), also known as DNMT2, is a DNA methyltransferase homolog that does not methylate DNA. Instead DNMT-2 methylates cytosine-38 in the anticodon loop of aspartic acid transfer RNA. Spec ifically, DNMT-2 methylates cytosine 38 in the anticodon loop of tRNA(Asp)

DNMT2 antibodies are useful for RNA research, cell replication studies, and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRDMT1