DNase II Antibody

Images

 
Western Blot: DNase II Antibody [NBP1-76971] - Human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: DNase II Antibody [NBP1-76971] - Staining of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml.
Immunohistochemistry: DNase II Antibody [NBP1-76971] - Staining of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml.
Western Blot: DNase II Antibody [NBP1-76971] - Analysis of Dnase II using NBP1-76971 at 2 ug/ml against 10 ug/lane of human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with ...read more
Immunohistochemistry-Paraffin: DNase II Antibody [NBP1-76971] - Human spleen tissue with DNaseII antibody at 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DNase II Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II.
Specificity
An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNASE2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunofluorescence
Application Notes
DNase II antibody can be used for detection of DNase II expression by Western blot at 1:500 to 1:1000 dilution. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DNase II Peptide (NBP1-76971PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNase II Antibody

  • Acid DNase
  • Deoxyribonuclease II alpha
  • deoxyribonuclease II, lysosomal
  • deoxyribonuclease-2-alpha
  • DNase II alpha
  • DNase II, lysosomal
  • DNASE2A
  • DNL
  • DNL2
  • EC 3.1.22.1
  • Lysosomal DNase II
  • R31240_2

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DNASE2
Entrez
Uniprot