DNAM-1/CD226 Antibody (BLR063G) - BSA Free

Western Blot: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Whole cell lysate (10 ug) from SUP-M2, RT-4, HH, U-251MG, MJ, HDLM-2, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .

Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.

Immunoprecipitation: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Detection of human DNAM-1/CD226 by WB of immunoprecipitates from HH lysate. Antibodies: Rabbit anti-DNAM-1/CD226 recombinant monoclonal [BLR063G] (NBP2-77357) and another rabbit anti-DNAM-1/CD226 recombinant monoclonal [BL-799B-1B9].

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: BLR063G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNAM-1/CD226 Antibody (BLR063G) - BSA Free Summary

• Description: Novus Biologicals Rabbit DNAM-1/CD226 Antibody (BLR063G) - BSA Free (NBP2-77357) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Immunogen was a recombinant protein representing the extracellular domain of human DNAM1/CD226.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD226
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for DNAM-1/CD226 Antibody (BLR063G) - BSA Free

• CD226 antigenplatelet and T cell activation antigen 1
• CD226 molecule
• CD226
• DNAM1
• DNAM-1
• DNAM1adhesion glycoprotein
• DNAM-1DNAX accessory molecule-1
• DNAX accessory molecule 1
• PTA1
• T lineage-specific activation antigen 1 antigen
• TLiSA1

Background

CD226 is a 65kD type I transmembrane glycoprotein, known as DNAM-1 (DNAX accessory molecule-1), PTA1(platelet and T cell activation antigen 1), or TLiSA1 (T lineage-specific activation antigen 1 antigen). It belongs to immunoglobulin superfamily containing 2 Ig-like domains, is expressed on majority of T lymphocytes, NK cells, monocytes, platelets, and a subset of B cells, activated endothelial cells. DNAM-1 is also expressed on CD8+ (but not CD8-) dendritic cells in mouse model. CD226 is an adhesion molecule involved in CTL and NK cell-mediated cytotoxicity. It has been identified that CD155 (poliovirus receptor, PVR) and CD112 (nectin-2, PRR-2) are CD226 ligands. DX11 antibody is able to inhibit CTL and NK cell-mediated cytotoxicity, and to block T cell cytokine production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD226