DNA-PKcs [p Ser2056] Antibody (4A4) - BSA Free

Immunocytochemistry/Immunofluorescence: DNA-PKcs [p Ser2056] Antibody (4A4) [NBP3-26398] - Staining of Hela cells(treated with UV) with DNA-PKcs [p Ser2056] Antibody (4A4) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 4A4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNA-PKcs [p Ser2056] Antibody (4A4) - BSA Free Summary

• Description: Novus Biologicals Rabbit DNA-PKcs [p Ser2056] Antibody (4A4) - BSA Free (NBP3-26398) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human DNA-PKcs [p Ser2056] [UniProt P78527]
• Modification: p Ser2056
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKDC
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DNA-PKcs [p Ser2056] Antibody (4A4) - BSA Free

• DNA-dependent protein kinase catalytic subunit-like
• DNA-dependent protein kinase
• DNA-PK catalytic subunit
• DNAPK
• DNAPKcs
• DNA-PKcs
• DNPK1
• EC 2.7.11.1
• HYRC
• HYRC1
• p350
• p460
• PRKDC
• XRCC7

Background

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKDC