Artemis Antibody

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Western Blot: Artemis Antibody [NB100-183] - Detection of Artemis protein in human testis lysate using NB 100-183.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Artemis Antibody Summary

Immunogen
A synthetic peptide made to residues 677-692 [GESIAVKKRKCSLLDT] of the human Artemis protein.
Specificity
Artemis
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCLRE1C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
Application Notes
This antibody is useful for Western blot. Weak staining is often seen due to low endogenous protein expression levels.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Testis Lysate (NB820-59266)
Artemis Lysate (NBL1-09743)
Reviewed Applications
Read 1 Review rated 3
using
NB100-183 in the following application:

Publications
Read Publication using
NB100-183 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reacts with human protein. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Artemis Antibody

  • ASCID
  • DCLREC1C
  • DNA cross-link repair 1C protein
  • DNA cross-link repair 1C
  • EC 3.1
  • FLJ36438
  • hSNM1C
  • protein artemis
  • Protein A-SCID
  • PSO2 homolog
  • RS-SCID
  • severe combined immunodeficiency, type a (Athabascan)
  • SNM1 homolog C
  • SNM1CS. cerevisiae)
  • SNM1-like protein

Background

Artemis is a novel protein that is involved in V(D)J recombination/DNA repair, Mutations cause human T-B-severe combined immunodeficiency associated with increased cellular radiosensitivity (RS-SCID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Artemis Antibody (NB100-183)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for Artemis Antibody (NB100-183) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-183:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Artemis NB100-183
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reviewed by:
Christie Brown
WB Human 08/15/2013
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Summary

ApplicationWestern Blot
Sample Testedprimary skin fibroblasts, whole cell lysate
SpeciesHuman
Lot5130
CommentsThis is not the cleanest antibody. There are several non-specific bands. Make sure you have an appropriate negative control. The correct band showed up at over 100 kDa in primary skin fibroblast lines - not sure if this is cell line specific. Artemis is expected to be 66 kDa.

Blocking

Blocking Details5% milk in PBSt

Primary Anitbody

Dilution Ratio1/500, overnight at 4C, 5% milk in PBSt

Secondary Antibody

Secondary DescriptionDonkey anti Rabbit IgG, HRP
Secondary Manufacturer Cat#45-000-682
Secondary Concentration1/3000

Details

Detection NotesAmersham ECL, 5 min exposure, wild type and Art-/- lysates

Comments

CommentsThis is not the cleanest antibody. There are several non-specific bands. Make sure you have an appropriate negative control. The correct band showed up at over 100 kDa in primary skin fibroblast lines - not sure if this is cell line specific. Artemis is expected to be 66 kDa.

Product General Protocols

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Video Protocols

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FAQs for Artemis Antibody (NB100-183) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Artemis Antibody (NB100-183)

Discover related pathways, diseases and genes to Artemis Antibody (NB100-183). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Artemis Antibody (NB100-183)

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Blogs on Artemis

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Recent Reviews

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1
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Christie Brown
08/15/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol DCLRE1C
Entrez