Ku70/XRCC6 Antibody (KU729)

Images

 
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent analysis of HeLa cells using Ku70/XRCC6 antibody (KU729). Image from verified customer review.
Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF700 (blue) and a matched isotype control (orange). Cells ...read more
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) ...read more
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Pm, Ch(-), Mu(-), Rt(-)Species Glossary
Applications Flow, Flow-IC, ICC/IF
Clone
KU729
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Ku70/XRCC6 Antibody (KU729) Summary

Immunogen
Nuclear extract of human HL-60 cells
Localization
Nuclear
Marker
Nuclear Marker
Specificity
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
XRCC6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1.0 ug/ml
Application Notes
Immunocytochemistry (Acetone-fixed cells): 0.5-1.0ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34247 in the following applications:

Reactivity Notes

Does not react with Mouse, Rat and Chicken.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34663)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Ku70/XRCC6 Antibody (KU729)

  • 5'-deoxyribose-5-phosphate lyase Ku70
  • 5'-dRP lyase Ku70
  • 70 kDa subunit of Ku antigen
  • ATP-dependent DNA helicase 2 subunit 1
  • ATP-dependent DNA helicase II 70 kDa subunit
  • CTC box binding factor 75 kDa subunit
  • CTC box-binding factor 75 kDa subunit
  • CTC75
  • CTCBF
  • D22S731
  • EC 3.6.4.-
  • EC 4.2.99.-
  • G22P1
  • G22P1Ku70
  • Ku autoantigen p70 subunit
  • Ku autoantigen, 70kDa
  • Ku70
  • ML8
  • ML8,70 kDa subunit
  • thyroid-lupus autoantigen p70
  • Thyroid-lupus autoantigen
  • TLAA
  • TLAAD22S671
  • X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6
  • X-ray repair cross-complementing protein 6
  • XRCC6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ku70/XRCC6 Antibody (NBP2-34247) (0)

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Review for Ku70/XRCC6 Antibody (NBP2-34247) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34247:
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Immunocytochemistry
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Ku70/XRCC6 NBP2-34247
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5
reviewed by:
Sarah Miller
Immunocytochemistry Human 03/22/2022
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Summary

ApplicationImmunocytochemistry
Sample TestedHeLa cells
SpeciesHuman
LotKU729

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Ku70/XRCC6 Antibody (NBP2-34247) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ku70/XRCC6 Antibody (NBP2-34247)

Discover related pathways, diseases and genes to Ku70/XRCC6 Antibody (NBP2-34247). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Sarah Miller
03/22/2022
Application: Immunocytochemistry
Species: Human

Bioinformatics

Gene Symbol XRCC6
Uniprot