DLEC/CLEC4C/BDCA-2 Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin

DLEC/CLEC4C/BDCA-2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DLEC/CLEC4C/BDCA‑2
  Phe46-Ile213
  Accession # Q8WTT0
• Specificity: Detects human DLEC/CLEC4C/BDCA‑2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLEC4C
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile Tris-buffered saline, pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2-4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5-8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5-9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).

1. Kanazawa, N. et al. (2004) Immunobiology 209:179.
2. Arce, I. et al. (2001) Eur. J. Immunol. 31:2733.
3. Dzionek, A. et al. (2001) J. Exp. Med. 194:1823.
4. Fernandes, M.J. et al. (2000) Genomics 69:263.
5. Wu, P. et al. (2008) Clin. Immunol. 129:40.
6. Fanning, S.L. et al. (2006) J. Immunol. 177:5829.
7. Rock, J. et al. (2007) Eur. J. Immunol. 37:3564.
8. Cao, W. et al. (2007) PloS Biol. 5:e248.
9. Riboldi, E. et al. (2009) Immunobiology 214:868.
10. Martinelli, E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3396.
11. Xu, Y. et al. (2009) Mol. Immunol. 46:2640.
12. Jaehn, P.S. et al. (2008) Eur. J. Immunol. 38:1822.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC4C