Dkk-1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Dkk-1 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
  Thr32-His266
  Accession # O94907
• Specificity: Detects human Dkk-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 85% cross‑reactivity with rmDkk-1 is observed and less than 0.2% cross‑reactivity with rhDkk-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DKK1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Dkk-1 Antibody (Catalog # MAB10962)
  ELISA Detection: Human Dkk-1 Biotinylated Antibody (Catalog # BAF1096)
  Standard: Recombinant Human Dkk-1 (Catalog # 5439-DK)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-1 Antibody [Biotin]

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is a member of the Dkk protein family that includes Dkk-1, -2, -3, and -4 (1). All four members are secreted proteins that are synthesized as precursor proteins with an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region. Dkk proteins have potential furin type proteolytic cleavage sites, and short forms of Dkk-2 and Dkk-4 containing only the second cysteine-rich domain can be generated by proteolytic processing (1). Dkk proteins have distinct patterns of expression in adult and embryonic tissues, suggesting that they may play diverse roles in these tissues. The Dkk proteins have distinct effects on Wnt signaling. Dkk-1 and Dkk-4 are Wnt antagonists. Dkk-3 has not been demonstrated to affect Wnt signaling, and Dkk-2 acts as an agonist or antagonist, depending on the cellular context. Wnt signaling regulates many important developmental processes including neural crest differentiation, brain development, kidney morphogenesis, and sex determination. In addition, Wnt signaling has also been implicated in tumor formation. Canonical Wnt signaling via the beta-catenin pathway is transduced by a receptor complex composed of the Frizzled proteins (Fz) and low-density lipoprotein (LDL) receptor-related proteins (LRP5/6) (2, 3). Unlike many soluble Wnt antagonists that function by binding extracellular Wnt ligands to prevent interaction of Wnt with the Fz-LRP5/6 receptor complex, Dkk-1 and Dkk-4 antagonize Wnt/ beta -catenin signaling by direct high-affinity binding to the Wnt coreceptor LRP5/6 and inhibiting interaction of LRP5/6 with the Wnt-Frizzled complex (4). Dkk-1 and Dkk-4 also bind the transmembrane proteins Kremen1 (Krm1) and Krm2 with high-affinity (5). Krm2 has been shown to form a ternary complex with Dkk-1 or -4 and LRP5/6 to trigger internalization of the complex and removal LRP6 from the cell surface. Thus, Dkk-1/4 and Kremens function synergistically to antagonize LRP5/6-mediated Wnt activity. Dkk-2 also binds to LRP5/6 and the Kremens, but Dkk-2 acts as antagonist of the Wnt signaling pathway only in the presence of Krm2 (5, 6). Dkk 2 binding to LRP5/6 in the absence of Krm2 activates rather than inhibits Wnt signaling (6).

1. Krupnik, V.E. et al. (1999) Gene 238:301.
2. Zorn, A.M (2001) Current Biology R592.
3. Mao, J. et al. (2001) Mol. Cell 7:801.
4. Nusse, R. et al. (2001) Nature 411:255.
5. Mao, J. et al. (2002) Nature 417:664.
6. Mao, B. and C. Niehrs (2003) Gene 302:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dkk-1 Antibody (BAF1096)(9)

Publications using BAF1096

• S Jumpertz, T Hennes, Y Asare, AK Schütz, J Bernhagen CSN5/JAB1 suppresses the WNT inhibitor DKK1 in colorectal cancer cells Cell. Signal, 2017-02-14;0(0):. 2017-02-14 [PMID: 28229932] (Western Blot, Human)
• Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al. Role of Wnt/ beta -catenin and RANKL/OPG in bone healing of diabetic Charcot arthropathy patients Acta Orthopaedica 7/21/2015 [PMID: 25811776]
• Gustafson B, Eliasson B, Smith U Thiazolidinediones increase the wingless-type MMTV integration site family (WNT) inhibitor Dickkopf-1 in adipocytes: a link with osteogenesis. Diabetologia, 2009-11-27;53(3):536-40. 2009-11-27 [PMID: 19943155] (ELISA Development, Human)
• Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WE Decreased osteogenesis, increased cell senescence and elevated Dickkopf-1 secretion in human fracture non union stromal cells. Bone, 2009-06-18;45(4):726-35. 2009-06-18 [PMID: 19540374] (ELISA Development, Human)
• Heider U, Kaiser M, Mieth M, Lamottke B, Rademacher J, Jakob C, Braendle E, Stover D, Sezer O Serum concentrations of DKK-1 decrease in patients with multiple myeloma responding to anti-myeloma treatment. Eur. J. Haematol., 2008-11-10;82(1):31-8. 2008-11-10 [PMID: 19067746] (ELISA Development, Human)
• Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma. Blood, 2008-02-27;112(1):196-207. 2008-02-27 [PMID: 18305214] (ELISA Development, Human)
• Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women. Arthritis Rheum., 2007-10-01;56(10):3319-25. 2007-10-01 [PMID: 17907185] (ELISA Development, Human)
• Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G Dickkopf-1 is a master regulator of joint remodeling. Nat. Med., 2007-01-21;13(2):156-63. 2007-01-21 [PMID: 17237793] (ELISA Development, Human)
• Naot D, Bava U, Matthews B, Callon KE, Gamble GD, Black M, Song S, Pitto RP, Cundy T, Cornish J, Reid IR Differential gene expression in cultured osteoblasts and bone marrow stromal cells from patients with Paget's disease of bone. J. Bone Miner. Res., 2007-02-01;22(2):298-309. 2007-02-01 [PMID: 17129176] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: DKK1
• Uniprot:
  • O94907()