Dkk-1 Antibody [Unconjugated]

Images

 
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Dkk‑1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat ...read more
Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF ...read more
Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, B/N, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Dkk-1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907
Specificity
Detects human and mouse Dkk-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Dkk-2, and rhDkk-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Blockade of Receptor-ligand Interaction
  • Knockout Validated
Control
Dkk-1 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF1096 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-1 Antibody [Unconjugated]

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is a member of the Dkk protein family that includes Dkk-1, -2, -3, and -4 (1). All four members are secreted proteins that are synthesized as precursor proteins with an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region. Dkk proteins have potential furin type proteolytic cleavage sites, and short forms of Dkk-2 and Dkk-4 containing only the second cysteine-rich domain can be generated by proteolytic processing (1). Dkk proteins have distinct patterns of expression in adult and embryonic tissues, suggesting that they may play diverse roles in these tissues. The Dkk proteins have distinct effects on Wnt signaling. Dkk-1 and Dkk-4 are Wnt antagonists. Dkk-3 has not been demonstrated to affect Wnt signaling, and Dkk-2 acts as an agonist or antagonist, depending on the cellular context. Wnt signaling regulates many important developmental processes including neural crest differentiation, brain development, kidney morphogenesis, and sex determination. In addition, Wnt signaling has also been implicated in tumor formation. Canonical Wnt signaling via the beta-catenin pathway is transduced by a receptor complex composed of the Frizzled proteins (Fz) and low-density lipoprotein (LDL) receptor-related proteins (LRP5/6) (2, 3). Unlike many soluble Wnt antagonists that function by binding extracellular Wnt ligands to prevent interaction of Wnt with the Fz-LRP5/6 receptor complex, Dkk-1 and Dkk-4 antagonize Wnt/ beta -catenin signaling by direct high-affinity binding to the Wnt coreceptor LRP5/6 and inhibiting interaction of LRP5/6 with the Wnt-Frizzled complex (4). Dkk-1 and Dkk-4 also bind the transmembrane proteins Kremen1 (Krm1) and Krm2 with high-affinity (5). Krm2 has been shown to form a ternary complex with Dkk-1 or -4 and LRP5/6 to trigger internalization of the complex and removal LRP6 from the cell surface. Thus, Dkk-1/4 and Kremens function synergistically to antagonize LRP5/6-mediated Wnt activity. Dkk-2 also binds to LRP5/6 and the Kremens, but Dkk-2 acts as antagonist of the Wnt signaling pathway only in the presence of Krm2 (5, 6). Dkk 2 binding to LRP5/6 in the absence of Krm2 activates rather than inhibits Wnt signaling (6).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.
  2. Zorn, A.M (2001) Current Biology R592.
  3. Mao, J. et al. (2001) Mol. Cell 7:801.
  4. Nusse, R. et al. (2001) Nature 411:255.
  5. Mao, J. et al. (2002) Nature 417:664.
  6. Mao, B. and C. Niehrs (2003) Gene 302:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dkk-1 Antibody (AF1096)(14)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 6 applications: Bioassay, ELISA Development, IHC, Immunodepletion, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(2)
IHC
(2)
Immunodepletion
(1)
Neutralization
(2)
Western Blot
(6)
All Applications
Filter By Species
Human
(13)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF1096 Applications Species
S Jumpertz, T Hennes, Y Asare, AK Schütz, J Bernhagen CSN5/JAB1 suppresses the WNT inhibitor DKK1 in colorectal cancer cells Cell. Signal, 2017;0(0):. 2017 [PMID: 28229932] (Bioassay, Human) Bioassay Human
Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells Nat Commun, 2016;7(0):13602. 2016 [PMID: 27934856] (Western Blot, Human) Western Blot Human
Zhang H, Yu C, Dai J, Keller J, Hua A, Sottnik J, Shelley G, Hall C, Park S, Yao Z, Zhang J, McCauley L, Keller E Parathyroid hormone-related protein inhibits DKK1 expression through c-Jun-mediated inhibition of beta-catenin activation of the DKK1 promoter in prostate cancer. Oncogene, 2014;33(19):2464-77. 2014 [PMID: 23752183] (Western Blot, Human) Western Blot Human
Ravindranath A, Yuen HF, Chan KK, Grills C, Fennell DA, Lappin TR, El-Tanani M Wnt-beta-catenin-Tcf-4 signalling-modulated invasiveness is dependent on osteopontin expression in breast cancer. Br. J. Cancer, 2011;105(4):542-51. 2011 [PMID: 21772333] (Western Blot, Human) Western Blot Human
Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 2011;145(6):926-40. 2011 [PMID: 21663795] (Neutralization, Human) Neutralization Human
Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. Int. J. Oncol., 2010;37(6):1495-501. 2010 [PMID: 21042718] (Western Blot, Human) Western Blot Human
Choi W, Wolber R, Gerwat W The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. J. Cell. Sci., 2010;123(0):3102-11. 2010 [PMID: 20736300] (Western Blot, Human) Western Blot Human
Lee N, Smolarz AJ, Olson S, David O, Reiser J, Kutner R, Daw NC, Prockop DJ, Horwitz EM, Gregory CA A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and treatment strategies. Br. J. Cancer, 2007;97(11):1552-9. 2007 [PMID: 17987039] (Immunodepletion, Human) Immunodepletion Human
Matushansky I, Hernando E, Socci ND, Mills JE, Matos TA, Edgar MA, Singer S, Maki RG, Cordon-Cardo C Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway. J. Clin. Invest., 2007;117(11):3248-57. 2007 [PMID: 17948129] (Western Blot, Human) Western Blot Human
Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women. Arthritis Rheum., 2007;56(10):3319-25. 2007 [PMID: 17907185] (ELISA Development, Human) ELISA Development Human
Show All 14 Publications.

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Control Lysate(s)

Secondary Antibodies

 

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Biotin BAF1096

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Blogs on Dkk-1.

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By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

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Bioinformatics

Gene Symbol DKK1
Uniprot