DIAPH-2 Antibody (699142)

Western blot shows lysates of human testis tissue and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human DIAPH-2 Monoclonal Antibody (Catalog # MAB6806) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIAPH-2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 699142
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DIAPH-2 Antibody (699142) Summary

• Immunogen: E. coli-derived recombinant human DIAPH-2
  Val314-Gln540
  Accession # O60879
• Specificity: Detects human DIAPH-2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DIAPH2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DIAPH-2 Antibody (699142)

• DIA2
• DIAdiaphanous (Drosophila, homolog) 2
• DIAPH2
• DIAPH-2
• diaphanous homolog 2 (Drosophila)
• Diaphanous-related formin-2
• diaphorase-2
• DRF2
• POF
• POF2
• POF2FLJ11167
• protein diaphanous homolog 2

Background

DIAPH2 (diaphenous homolog 2), also called DRF2 (diaphanous-related formin 2), is an 1101 amino acid (aa), 126 kDa (calculated) cytoplasmic protein in the Diaphenous family of actin binding proteins. Disruption of DIAPH2 has been correlated with premature ovarian failure in humans. Within the region used as an immunogen, human DIAPH2 shares 89% and 90% aa identity with mouse and rat DIAPH2, respectively. Two additional isoforms have been described. One, called hDia2C (1097 aa), is lacking aa 45-55, but has 7 aa inserted after aa 149. This form specifically binds RhoD and restricts movement of early endosomes along actin filaments. A 1106 aa form diverges after aa 1080.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIAPH2
• Uniprot:
  • O60879()