DGAT1 Antibody

Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB100-57086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DGAT1 Antibody Summary

• Description: Novus Biologicals Goat DGAT1 Antibody (NB100-57086) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-DGAT1 Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QNSMKPFKDMDYS corresponding to internal region according to NP_036211.1.
• Epitope: QNSMKPFKDMDYS
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DGAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.1 ug/mL
• Application Notes: WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 55.3 kDa band according to NP_036211.1).
• Theoretical MW: 55.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Duodenum Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25714675).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DGAT1 Antibody

• ACAT-related gene product 1
• AGRP1
• ARAT
• ARGP1
• ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1
• DGAT
• DGAT1
• DGATACAT related gene product 1
• diacylglycerol O-acyltransferase 1
• diacylglycerol O-acyltransferase homolog 1 (mouse)
• diacylglycerol O-acyltransferase homolog 1
• DIAR7
• Diglyceride acyltransferase
• EC 2.3.1
• EC 2.3.1.20

Background

The enzyme encoded by this gene utilizes diacylglycerol and fatty acyl CoA as substrates in order to catalyze the final stage of triacylglycerol synthesis. It is also involved in cellular as well as physiological metabolic processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGAT1 Antibody (NB100-57086)(10)

Publications using NB100-57086

• Chaves AB, Zheng D, Johnson JA et al. Infant Mesenchymal Stem Cell Insulin Action is Associated With Maternal Plasma Free Fatty Acids, Independent of Obesity Status: The Healthy Start Study Diabetes 2022-05-27 [PMID: 35621990]
• Melissa L. Erickson, Zachary W. Patinkin, Allison M. Duensing, Dana Dabelea, Leanne M. Redman, Kristen E. Boyle Maternal metabolic health drives mesenchymal stem cell metabolism and infant fat mass at birth JCI Insight 2021-07-08 [PMID: 34061777]
• Timothy M. Moore, Zhenqi Zhou, Alexander R. Strumwasser, Whitaker Cohn, Amanda J. Lin, Kevin Cory, Kate Whitney, Theodore Ho, Timothy Ho, Joseph L. Lee, Daniel H. Rucker, Austin N. Hoang, Kevin Widjaja, Aaron D. Abrishami, Sarada Charugundla, Linsey Stiles, Julian P. Whitelegge, Lorraine P. Turcotte, Jonathan Wanagat, Andrea L. Hevener Age‐induced mitochondrial DNA point mutations are inadequate to alter metabolic homeostasis in response to nutrient challenge Aging Cell 2020-10-13 [PMID: 33049094]
• Larsen Steen, Vigelso Andreas, Dandanell Sune et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. J Diabetes Res. 2018-09-12 [PMID: 30276217]
• Borsting Jordy A, Kraakman MJ, Gardner T et al. Analysis of the liver lipidome reveals insights into the protective effect of exercise on high fat diet induced hepatosteatosis in mice Am. J. Physiol. Endocrinol. Metab. 2015-02-24 [PMID: 25714675] (WB, Mouse)
• Seyer A, Cantiello M, Bertrand-Michel J et al. Lipidomic and Spatio-Temporal Imaging of Fat by Mass Spectrometry in Mice Duodenum during Lipid Digestion PLoS One 2013-01-01 [PMID: 23560035] (WB, Mouse)
• van Diepen JA, Stienstra R, Vroegrijk IO et al. Caspase-1 deficiency in mice reduces intestinal triglyceride absorption and hepatic triglyceride secretion. J Lipid Res 2013-02-01 [PMID: 23160218]
• Amati F, Dube JJ, Alvarez-Carnero E et al. Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: another paradox in endurance-trained athletes? Diabetes 2011-10-01 [PMID: 21873552] (WB, Human)
• Alsted TJ, Nybo L, Schweiger M et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training. Am J Physiol Endocrinol Metab;296(3):E445-453. 2009-01-01 [PMID: 19106247]
• Bagnato C, Igal RA. Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts. J Biol Chem 2003-12-26 [PMID: 14557275]

Bioinformatics

• Gene Symbol: DGAT1
• Entrez:
  • 8694(Human)
  • 13350(Mouse)
  • 84497(Rat)
• Uniprot:
  • O75907(Human)