Desmin Antibody (SI18-00)

Western Blot: Desmin Antibody (SI18-00) [NBP2-67407] - Western blot analysis of Desmin on rat skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 ho

Immunocytochemistry/Immunofluorescence: Desmin Antibody (SI18-00) [NBP2-67407] - Staining Desmin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Desmin Antibody (SI18-00) [NBP2-67407] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Desmin antibody. Counter stained with hematoxylin.

Flow Cytometry: Desmin Antibody (SI18-00) [NBP2-67407] - Analysis of C2C12 cells with Desmin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Desmin Antibody (SI18-00) [NBP2-67407] - Analysis of Desmin on different lysates using anti-Desmin antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse heart

Immunohistochemistry-Paraffin: Desmin Antibody (SI18-00) [NBP2-67407] - Analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmin Antibody (SI18-00) [NBP2-67407] - Analysis of paraffin-embedded mouse bladder tissue using anti-Desmin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmin Antibody (SI18-00) [NBP2-67407] - Analysis of paraffin-embedded mouse heart tissue using anti-Desmin antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SI18-00
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Desmin Antibody (SI18-00) Summary

• Description: Novus Biologicals Rabbit Desmin Antibody (SI18-00) (NBP2-67407) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Desmin aa 421-470 / 470. (SwissProt: P17661 Human; SwissProt: P31001 Mouse; SwissProt: P48675 Rat)
• Localization: Cytoplasm, Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DES
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Desmin Antibody (SI18-00)

• CMD1I
• CMD1IFLJ41013
• CSM1
• CSM2
• DES
• Desmin
• FLJ12025
• FLJ39719
• FLJ41793
• intermediate filament protein

Background

Desmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle.DE-U-10 labels desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas) (Debus E et al.).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DES