Dectin-2/CLEC6A Antibody [Unconjugated] - a Isoform

Raw264.7 cells were stained with Goat Anti-Mouse Dectin‑2/CLEC6A alpha Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1525, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Dectin-2/CLEC6A Antibody [Unconjugated] - a Isoform Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑2/CLEC6A
  Phe43-Leu209
  Accession # Q9JKF4
• Specificity: Detects mouse Dectin‑2/CLEC6A alpha Isoform in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DLEC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Clec4n
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-2/CLEC6A Antibody [Unconjugated] - a Isoform

• CLEC4N
• CLEC6A
• CLECSF10
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
• C-type lectin domain family 6 member A
• C-type lectin domain family 6, member A
• C-type lectin superfamily member 10
• DC-associated C-type lectin 2
• dectin 2
• Dectin2
• Dectin-2
• Dendritic cell-associated C-type lectin 2

Background

Dectin-2 alpha , also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha , CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4⁺CD25⁺ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dectin-2/CLEC6A Antibody (AF1525)(1)

Publication using AF1525

• The innate immune receptor Dectin-2 mediates the phagocytosis of cancer cells by Kupffer cells for the suppression of liver metastasis Proc. Natl. Acad. Sci. U.S.A., 2016-11-21;113(49):14097-14102. 2016-11-21 [PMID: 27872290] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Clec4n
• Uniprot:
  • Q9JKF4()