CLEC4E Antibody (16E3)

Images

 
Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human MINCLE antibody (1:1000). Proteins were visualized using a ...read more
Immunocytochemistry/ Immunofluorescence: CLEC4E Antibody (16E3) [NBP1-49311] - HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) [NBP1-49311] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen ...read more
Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - Flow cytometry analysis of CLEC4E in LNCap cell line,staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 ...read more
Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CLEC4E (1:1000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
16E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CLEC4E Antibody (16E3) Summary

Immunogen
Recombinant human CLEC4E (41-219aa) purified from E. coli (NP_055173).
Specificity
Human CLEC4E (41-219aa)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CLEC4E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:250-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-49311.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CLEC4E Antibody (16E3)

  • CLEC4E
  • CLECSF9
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9
  • C-type lectin domain family 4 member E
  • C-type lectin domain family 4, member E
  • Macrophage-inducible C-type lectin
  • MINCLE
  • MINCLEC-type lectin superfamily member 9

Background

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor ? chain (FcR?) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CLEC4E Antibody (NBP1-49311)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49311AF405
Alexa Fluor 488 Labeled NBP1-49311AF488
Alexa Fluor 647 Labeled NBP1-49311AF647
Alexa Fluor 700 Labeled NBP1-49311AF700
DyLight 350 Labeled NBP1-49311UV
DyLight 405 Labeled NBP1-49311V
DyLight 488 Labeled NBP1-49311G
DyLight 680 Labeled NBP1-49311FR
DyLight 755 Labeled NBP1-49311IR

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Bioinformatics Tool for CLEC4E Antibody (NBP1-49311)

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Bioinformatics

Gene Symbol CLEC4E
Entrez
Uniprot