DCIR/CLEC4A Antibody (111F8.04) - Azide and BSA Free

Immunohistochemistry: DCIR/CLEC4A Antibody (111F8.04) [DDX0180P-100] - IHC staining of human tonsil frozensection with 111F8 (X1000)

Flow Cytometry: DCIR/CLEC4A Antibody (111F8.04) [DDX0180P-100] - FACS staining of monocytederivedDCs(GM+IL4) with111F8

Immunohistochemistry-Paraffin: DCIR/CLEC4A Antibody (111F8.04) [DDX0180P-100] - C-type lectin with a tyrosine based inhibitory motif

Flow (Cell Surface): DCIR/CLEC4A Antibody (111F8.04) [DDX0180P-100] - Analysis using the PE conjugate of DDX0180P-100. Staining of DCIR (shaded histogram) in human PBMCs using the monocyte population gated using forward and side scatter with DDX0180PE at 1ug/ml (A) and 3 ug/ml (B). Open histogram is isotype control.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 111F8.04
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

DCIR/CLEC4A Antibody (111F8.04) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DCIR/CLEC4A Antibody (111F8.04) - Azide and BSA Free (DDX0180P-100) is a monoclonal antibody validated for use in IHC and Flow. Anti-DCIR/CLEC4A Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DCIR was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC4A
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for DCIR/CLEC4A Antibody (111F8.04) - Azide and BSA Free

• CD367
• CLEC4A
• CLECSF6
• CLECSF6C-type lectin superfamily member 6
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 6
• C-type lectin domain family 4 member A
• C-type lectin domain family 4, member A
• DCIR
• DCIRLLIR
• DDB27
• DDB27C-type lectin DDB27
• Dendritic cell immunoreceptor
• Lectin-like immunoreceptor
• LLIR

Background

CLEC4A, also known as DCIR, is a member of the C-type lectin/C-type lectin-like (CTL/CTLD) domain superfamily. Members of the CTL/CTLD family have a variety of functions including cell adhesion, glycoprotein turnover and cell-cell signaling. CLEC4A may play a role in regulating immune reactivity. This protein is currently being studied for research on the following diseases and disorders: rheumatoid arthritis, osteoarthritis, pneumonia, influenza, eclampsia and melanoma. CLEC4A is known to have interactions with PTPN6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCIR/CLEC4A Antibody (DDX0180P-100)(10)

Publications using DDX0180P-100

• Kuipers ME, Nolte-'t Hoen ENM, van der Ham AJ et al. DC-SIGN mediated internalisation of glycosylated extracellular vesicles from Schistosoma mansoni increases activation of monocyte-derived dendritic cells J Extracell Vesicles 2020-04-30 [PMID: 32489529] (Human)
• Clark GJ et al. New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology 2016-01-01 [PMID: 26900474]
• Ohradanova-Revic A et al. Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel. Clin Transl Immunology 2016-01-01 [PMID: 26900469]
• Autenrieth SE et al. Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Transl Immunology 2015-11-01 [PMID: 26682057]
• Garcia-Vallejo JJ et al. The consequences of multiple simultaneous C-type lectin-ligand interactions: DCIR alters the endo-lysosomal routing of DC-SIGN. Front Immunol 2015-03-01 [PMID: 25806031]
• Tel J et al. Targeting uptake receptors on human plasmacytoid dendritic cells triggers antigen cross-presentation and robust type I IFN secretion. J Immunol 2013-11-01 [PMID: 24127556]
• Bloem K et al. Ligand binding and signaling of dendritic cell immunoreceptor (DCIR) is modulated by the glycosylation of the carbohydrate recognition domain. PLoS One 2013-06-01 [PMID: 23776650]
• Bloem K et al. Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells. Front Immunol 2013-05-01 [PMID: 23653626]
• Tjomsland V et al. Semi mature blood dendritic cells exist in patients with ductal pancreatic adenocarcinoma owing to inflammatory factors released from the tumor. PLoS One 2010-10-01 [PMID: 20976171]
• Bates EE et al. APCs express DCIR, a novel C-type lectin surface receptor containing an immunoreceptor tyrosine-based inhibitory motif. J Immunol 1999-08-01 [PMID: 10438934]

Bioinformatics

• Gene Symbol: CLEC4A