Dectin-1/CLEC7A Antibody

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Dectin-1/CLEC7A Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A (R&D Systems, Catalog # 1859-DC)
  Thr66-Met201
  Accession # NP_072092
• Specificity: Detects human Dectin-1/CLEC7A in direct ELISAs and Western blots. In this format, less than 2% cross‑reactivity with recombinant mouse Dectin-1 and recombinant human Dectin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLEC7A
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <2.0 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-1/CLEC7A Antibody

• Beta-glucan receptor
• BGR
• CD369
• CLEC7A
• CLECSF12
• CLECSF12DC-associated C-type lectin 1
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
• C-type lectin domain family 7 member A
• C-type lectin domain family 7, member A
• C-type lectin superfamily member 12
• Dectin1
• Dectin-1
• DECTIN1CANDF4
• Dendritic cell-associated C-type lectin 1
• dendritic cell-associated C-type lectin-1
• hDectin-1
• lectin-like receptor 1

Background

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
4. Yokota, K. et al. (2001) Gene 272:51.
5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dectin-1/CLEC7A Antibody (AF1859)(8)

Publications using AF1859

• S Haji, T Ito, C Guenther, M Nakano, T Shimizu, D Mori, Y Chiba, M Tanaka, SK Mishra, JA Willment, GD Brown, M Nagae, S Yamasaki Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2 Elife, 2022-12-08;11(0):. 2022-12-08 [PMID: 36479973] (Western Blot, Mouse)
• JS Griffiths, A Thompson, M Stott, A Benny, NA Lewis, PR Taylor, J Forton, S Herrick, SJ Orr, EP McGreal Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases FASEB J., 2018-01-22;0(0):fj201701145R. 2018-01-22 [PMID: 29401615] (Western Blot, Human)
• G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 2018-02-26;0(0):. 2018-02-26 [PMID: 29359350] (Neutralization, Human)
• Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan. J Immunol, 2013-03-20;190(8):4116-28. 2013-03-20 [PMID: 23514738] (Western Blot, Human, Mouse)
• Rizzetto , Lisa, Buschow , Sonja I, Beltrame , Luca, Figdor , Carl G, Schierer , Stephan, Schuler , Gerold, Cavalieri , Duccio The modular nature of dendritic cell responses to commensal and pathogenic fungi. PLoS ONE, 2012-08-03;7(8):e42430. 2012-08-03 [PMID: 22879980] (Western Blot, Human)
• Eva M. Carmona, Theodore J. Kottom, Deanne M. Hebrink, Teng Moua, Raman-Deep Singh, Richard E. Pagano et al. Glycosphingolipids Mediate Pneumocystis Cell Wall beta -Glucan Activation of the IL-23/IL-17 Axis in Human Dendritic Cells American Journal of Respiratory Cell and Molecular Biology 7/1/2012 [PMID: 22343219]
• Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes. J. Leukoc. Biol., 2011-06-07;90(3):599-611. 2011-06-07 [PMID: 21653233] (Western Blot, Human)
• Valera I, Fernandez N, Trinidad AG, Alonso S, Brown GD, Alonso A, Crespo MS Costimulation of dectin-1 and DC-SIGN triggers the arachidonic acid cascade in human monocyte-derived dendritic cells. J. Immunol., 2008-04-15;180(8):5727-36. 2008-04-15 [PMID: 18390758] (ICC, Western Blot, Human)

Review for Dectin-1/CLEC7A Antibody (AF1859) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24068891
• Species: Human

Bioinformatics

• Gene Symbol: CLEC7A
• Uniprot:
  • NP_072092()