Dectin-1/CLEC7A Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Dectin-1/CLEC7A Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A (R&D Systems, Catalog # 1859-DC)
Thr66-Met201
Accession # NP_072092
Specificity
Detects human Dectin-1/CLEC7A in direct ELISAs and Western blots. In this format, less than 2% cross‑reactivity with recombinant mouse Dectin-1 and recombinant human Dectin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLEC7A
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<2.0 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 1 Review rated 4
using
AF1859 in the following applications:

Publications
Read Publications using
AF1859 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-1/CLEC7A Antibody

  • Beta-glucan receptor
  • BGR
  • CD369
  • CLEC7A
  • CLECSF12
  • CLECSF12DC-associated C-type lectin 1
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
  • C-type lectin domain family 7 member A
  • C-type lectin domain family 7, member A
  • C-type lectin superfamily member 12
  • Dectin1
  • Dectin-1
  • DECTIN1CANDF4
  • Dendritic cell-associated C-type lectin 1
  • dendritic cell-associated C-type lectin-1
  • hDectin-1
  • lectin-like receptor 1

Background

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dectin-1/CLEC7A Antibody (AF1859)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(1)
Western Blot
(5)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF1859 Applications Species
JS Griffiths, A Thompson, M Stott, A Benny, NA Lewis, PR Taylor, J Forton, S Herrick, SJ Orr, EP McGreal Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases FASEB J., 2018;0(0):fj201701145R. 2018 [PMID: 29401615] (Western Blot, Human) Western Blot Human
G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 2018;0(0):. 2018 [PMID: 29359350] (Neutralization, Human) Neutralization Human
Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan. J Immunol, 2013;190(8):4116-28. 2013 [PMID: 23514738] (Western Blot, Mouse) Western Blot Mouse
Rizzetto, Lisa, Buschow, Sonja I, Beltrame, Luca, Figdor, Carl G, Schierer, Stephan, Schuler, Gerold, Cavalieri, Duccio The modular nature of dendritic cell responses to commensal and pathogenic fungi. PLoS ONE, 2012;7(8):e42430. 2012 [PMID: 22879980] (Western Blot, Human) Western Blot Human
Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes. J. Leukoc. Biol., 2011;90(3):599-611. 2011 [PMID: 21653233] (Western Blot, Human) Western Blot Human
Valera I, Fernandez N, Trinidad AG, Alonso S, Brown GD, Alonso A, Crespo MS Costimulation of dectin-1 and DC-SIGN triggers the arachidonic acid cascade in human monocyte-derived dendritic cells. J. Immunol., 2008;180(8):5727-36. 2008 [PMID: 18390758] (Western Blot, Human) Western Blot Human

Review for Dectin-1/CLEC7A Antibody (AF1859) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1859:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IF Human 01/05/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24068891
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24068891

Details

Detection NotesSee PMID 24068891

Comments

CommentsPublished: See PMID 24068891

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Biotin BAF1859

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Anonymous
01/05/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol CLEC7A
Uniprot