DDX54 Antibody (2H6) - Azide and BSA Free

Western Blot: DDX54 Antibody (2H6) [H00079039-M01] - DDX54 monoclonal antibody (M01), clone 2H6 Analysis of DDX54 expression in HeLa.

Immunocytochemistry/Immunofluorescence: DDX54 Antibody (2H6) [H00079039-M01] - Analysis of monoclonal antibody to DDX54 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: DDX54 Antibody (2H6) [H00079039-M01] - Analysis of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.

Western Blot: DDX54 Antibody (2H6) [H00079039-M01] - Analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody (M01), clone 2H6.Lane 1: DDX54 transfected lysate(98.6 KDa).Lane 2: Non-transfected lysate.

ELISA: DDX54 Antibody (2H6) [H00079039-M01] - Detection limit for recombinant GST tagged DDX54 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DDX54 Antibody (2H6) - Azide and BSA Free Summary

• Immunogen: DDX54 (NP_076977, 778 a.a. ~ 881 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDRDSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLHFLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM
• Specificity: DDX54 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX54
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DDX54 Antibody (2H6) - Azide and BSA Free

• APR-5
• ATP-dependent RNA helicase DP97
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
• DEAD box helicase 97 KDa
• DEAD box protein 54
• DEAD box RNA helicase 97 kDa
• DP97ATP-dependent RNA helicase DDX54
• EC 3.6.1
• EC 3.6.4.13
• MGC2835

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX54
• Entrez:
  • 79039(Human)
• Uniprot:
  • NP_076977(Human)