DDX5 Antibody (2C3) - BSA Free

Immunohistochemistry: DDX5 Antibody (2C3) [NBP3-26627] - Image of DDX5 Antibody (2C3) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: DDX5 Antibody (2C3) [NBP3-26627] - Positive Western Blot detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue. All lanes: DDX5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70, 61 kDa Observed band size: 70 kDa

Immunocytochemistry/Immunofluorescence: DDX5 Antibody (2C3) [NBP3-26627] - Staining of HepG2 Cells with DDX5 Antibody (2C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: DDX5 Antibody (2C3) [NBP3-26627] - Overlay histogram showing Hela cells stained with DDX5 Antibody (2C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 2C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDX5 Antibody (2C3) - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX5 Antibody (2C3) - BSA Free (NBP3-26627) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human DDX5 [UniProt P17844]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDX5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:20-1:200
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DDX5 Antibody (2C3) - BSA Free

• B-cell CLL/lymphoma 7 protein family member C
• B-cell CLL/lymphoma 7C
• DDX5
• DEAD/H
• G17P1
• HELR
• HLR1
• HUMP68

Background

DDX5, also known as Probable ATP-dependent RNA helicase DDX5, is a 69.1 kDa, 613 amino acid protein that regulates pre-mRNA splicing by increasing the inclusion of tau exon 10. Current research with the protein is being conducted for diseases and disorders such as squamous cell carcinoma, breast cancer, fibrosis, prostate cancer, hepatitis, malaria, and myeloma. The protein interacts in translational control and spliceosome pathways with HIST1H4A- HIST1H4E proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX5