DDX18 Antibody

Western Blot: DDX18 Antibody [NB100-1722] - Detection of Human DDX18 on HeLa whole cell lysate using NB100-1722. DDX18 was also immunoprecipitated using rabbit anti-DDX18 antibody NB100-1721.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX18 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 625 and the C-terminus (residue 670) of human DEAD (Asp-Glu-Ala-Asp) Box Protein 18 using the numbering given in entry NP_006764.3 (GeneID 8886).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Control:

  DDX18 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX18 Antibody

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
• DEAD box protein 18
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated)
• EC 3.6.1
• EC 3.6.4.13
• FLJ33908
• MrDbATP-dependent RNA helicase DDX18
• Myc-regulated DEAD box protein

Background

DDX18 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX18 has been observed to be a target of myc-max heterodimers. Alternate names for DDX18 include ATP-dependent RNA helicase DDX18, DEAD box protein 18, myc-regulated DEAD box protein, and MrDb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX18 Antibody (NB100-1722)(1)

Publication using NB100-1722

• Payne EM, Bolli N, Rhodes J et al. Ddx18 is essential for cell-cycle progression in zebrafish hematopoietic cells and is mutated in human AML. Blood 2011-07-01 [PMID: 21653321]

Bioinformatics

• Gene Symbol: DDX18
• Entrez:
  • 8886(Human)
• Uniprot:
  • NP_006764.3(Human)