RUVBL1 Antibody

Images

 
Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Paraffin-embedded mouse testis. RUVBL1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Paraffin-embedded rat heart. RUVBL1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Paraffin-embedded mouse colon. RUVBL1 antibody diluted at 1:500
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Paraffin-embedded rat testis. RUVBL1 antibody diluted at 1:500.
Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Pm, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RUVBL1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.
Localization
Nucleus matrix; Nucleus; nucleoplasm; Cytoplasm; Membrane
Predicted Species
Bovine (99%), Rhesus Macaque (100%), Zebrafish (97%), Xenopus (97%), Primate (100%), Chicken (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RUVBL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20245 in the following applications:

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for RUVBL1 Antibody

  • 49 kDa TATA box-binding protein-interacting protein
  • 54 kDa erythrocyte cytosolic protein
  • EC 3.6.1
  • EC 3.6.4.12,49 kDa TBP-interacting protein
  • ECP54
  • INO80 complex subunit H
  • INO80HTIP49A
  • NMP 238
  • NMP238ECP-54
  • Nuclear matrix protein 238
  • Pontin 52
  • PONTIN
  • Pontin52
  • RuvB (E coli homolog)-like 1
  • RuvB-like 1 (E. coli)
  • ruvB-like 1
  • RVB1
  • TATA binding protein interacting protein 49 kDa
  • TIH1
  • TIP49a
  • TIP49TAP54-alpha
  • TIP60-associated protein 54-alpha

Background

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUVBL1 Antibody (NBP2-20245) (0)

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Review for RUVBL1 Antibody (NBP2-20245) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-20245:
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Images Ratings Applications Species Date Details
Immunoprecipitation RUVBL1 NBP2-20245
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reviewed by:
Tetsuo Kiguchi
IP Human 11/30/2017
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Summary

ApplicationImmunoprecipitation
Sample TestedCancer Cell lysate
SpeciesHuman
Lot42662

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Tetsuo Kiguchi
11/30/2017
Application: IP
Species: Human

Bioinformatics

Gene Symbol RUVBL1
Uniprot