Western Blot: RUVBL1 Antibody [NBP2-20245] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:2500. ...read more
Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Paraffin-embedded rat testis. RUVBL1 antibody diluted at 1:500.
Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot ...read more
Use in Knockdown Validated reported in scientific literature (PMID: 31018511).
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 4.5 using NBP2-20245 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for RUVBL1 Antibody
49 kDa TATA box-binding protein-interacting protein
54 kDa erythrocyte cytosolic protein
EC 18.104.22.168,49 kDa TBP-interacting protein
INO80 complex subunit H
Nuclear matrix protein 238
RuvB (E coli homolog)-like 1
RuvB-like 1 (E. coli)
TATA binding protein interacting protein 49 kDa
TIP60-associated protein 54-alpha
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.